HPLC-DAD-MS研究黄芪的化学成分  

Chemical composition analysis of Astragali Radix by HPLC-DAD-MS

作　　者：王平[1,2] 梁逸曾[2] 

机构地区：[1]大连医科大学,辽宁大连116044 [2]中南大学,湖南长沙410083

出　　处：《中草药》2011年第2期226-229,共4页Chinese Traditional and Herbal Drugs

基　　金：辽宁省教育厅科研计划资助项目(2008174)

摘　　要：目的建立HPLC-DAD-MS快速分离分析黄芪Astragali Radix化学成分的方法。方法色谱柱为Thermo Hypersil-Hypurity C18(150 mm×2.1 mm,5μm)分析柱,柱温40℃,流动相水-甲醇(梯度洗脱),体积流量0.25 mL/min。二极管阵列扫描范围200～370 nm。质谱采用大气压化学电离(APCI+)离子源,扫描范围m/z 100～650。结果通过与已有文献报道的质谱、紫外光谱和保留行为比较可以初步定性5个化合物,分别为calycosin-7-O-β-D-glycoside、ononin、calycosin、formononetin、(3R)-7,2′-dihydroxy-3′,4′-dimethoxyisoflavan。结论因为能够同时提供相对分子质量、紫外光谱和保留行为,所以HPLC-DAD-MS是一种分析中药化学成分的有力方法。Objective To study a method of chemical constituents of Astragali Radix by HPLC-DAD-MS.Methods The compounds were separated by using Thermo Hypersil-Hypurity C18（150 mm×2.1 mm,5 μm） analytical column.The oven temperature was adjusted at 40 ℃.Mobile phase consisted of methanol and water using a gradient elution.The flow rate was set at 0.25 mL/min.UV spectra were obtained by scanning from 200 to 370 nm.The APCI-MS spectra were obtained in the positive ion mode scanning from m/z 100 to 650.Results The peaks can be identified by comparing their UV spectra,the molecular weights and retention behaviors with data reported in the literature.At least five compounds were tentatively identified as calycosin-7-O-β-D-glycoside,ononin,calycosin,formononetin,and（3R）-7,2′-dihydroxy-3′,4′-dimethoxyisoflavan.Conclusion Because of providing UV and molecular mass as well as retention time,HPLC-DAD-MS is a powerful tool for chemical analysis of traditional Chinese material medica.

关 键 词：黄芪 HPLC-DAD-MS calycosin-7-O-β-D-glycoside (3R)-7 2′-dihydroxy-3′ 4′-dimethoxyisoflavan CALYCOSIN 

分 类 号：R284.1[医药卫生—中药学]