中西药联合对胃癌SGC-7901/ADR细胞MRP介导的耐药性研究  

Research on MRP Mediated Multi-drug Resistance in Gastric Carcinoma SGC-7901/ADR Cells by Combination of Modern Medicine and Traditional Chinese Medicine

作　　者：郝海燕[1] 侯培珍[1] 

机构地区：[1]包头医学院第一附属医院,内蒙古包头014010

出　　处：《包头医学院学报》2011年第1期5-7,共3页Journal of Baotou Medical College

摘　　要：目的:探讨中西药联合逆转胃癌多药耐药亚系SGC-7901/ADR细胞MRP介导的耐药性作用。方法:以SGC-7901/ADR细胞为靶细胞,以不加药组为阴性对照组,5-FU组、斑贞1号组、班贞1号+5-FU组、班贞1号+5-FU+TMP组为实验组,采用半定量RT-PCR法检测SGC-7901/ADR细胞在不同药物作用下MRP mRNA的表达情况。结果:5-FU组与阴性对照组比较,耐药细胞MRP mRNA表达差异无统计学意义(P>0.05),提示MRP参与SGC-7901/ADR细胞对5-FU的多药耐药性;其他实验组组间比较及与阴性对照组比较,MRP mRNA表达差异均有统计学意义(P<0.01),中西药联合组(斑贞1号+5-FU+TMP)MRP mRNA表达量最低(仅为0.07)。结论:中西药联合可以克服SGC-7901/ADR细胞由MRP介导的多药耐药性,为当前胃癌的治疗提供了新的理论依据。Objective： To investigate the effects of combination of modern medicine and traditional Chinese medicine on MRP mediated multi-drug resistance in gastric carcinoma SGC-7901/ADR cells.Methods： The SGC-7901/ADR cells were regarded as target cells,no medicine added group were considered as negative control,5-FU,Banzhen-1,Banzhen-1＋5-FU,Banzhen-1＋5-FU＋TMP,were added to the target cells,respectively,and were considered as experimental groups.The expression of MRP mRNA in the SGC-7901/ADR cells was detected by the semi-quantitative RT-PCR.Results： No significant difference in the expression of MRP mRNA was detected between the 5-FU group and the negative controls（P0.05）,suggesting MRP was involved in 5-FU resistance in SGC-7901/ADR cells.However,the significant difference in the expression of MRP mRNA was seen between the other experimental groups and the negative controls,as well as among the different experimental groups（P﹤0.01）.The expression in the group of Banzhen-1＋5-FU＋TMP was the lowest（only 0.07）.Conclusion： The combination of modern medicine and traditional Chinese medicine could overcome the problem of MRP mediated multi-drug resistance in gastric carcinoma SGC-7901/ADR cells,which might supply a new theoretical basis for the treatment of gastric cancer.

关 键 词：中西药结合 胃癌 多药耐药 MRP基因 细胞培养 

分 类 号：R735.2[医药卫生—肿瘤]