牛附红细胞体16SrRNA基因的克隆测序及分析  

Cloning and sequence analysis of the 16S rRNA gene of Eperythrozoon wenyonii in cow

作　　者：周作勇[1] 聂奎[2] 胡世君[1] 周荣琼[1] 唐成 

机构地区：[1]西南大学荣昌校区动物医学系,重庆荣昌402460 [2]西南大学动物科技学院,重庆北碚400715 [3]四川省遂宁市船山区畜牧食品局,四川遂宁629000

出　　处：《中国兽医学报》2011年第3期329-333,341,共6页Chinese Journal of Veterinary Science

基　　金：重庆市自然科学基金计划重点资助项目(CSTC;2006BA1009)

摘　　要：无菌采集自然感染附红细胞体的牛血液,提取全血基因组,用血营养菌16S rRNA基因的通用引物进行PCR扩增,得到长约1500 bp的扩增片段,将其克隆到pMD18-T载体后进行测序和分析。结果,所克隆的牛附红细胞体基因片段大小为1 454 bp,GenBank登录号为FJ375309(丰都株)。序列比对结果显示,牛附红细胞体丰都株与武汉株(AY946266)最高,达99.7%,与支原体科代表种同源性为60.7%～76.2%,而与立克次氏体科的立克次氏体和无形体科的无形体同源性仅为51.4%～56.4%,表明牛附红细胞体应归为支原体科,附红细胞体属,而不应属于立克次氏体目,无浆体科。对国内外牛温氏附红细胞体的亲缘关系分析表明,牛温氏附红细胞体无明显的地域性差别趋势。we cloned and sequenced 16S rRNA gene of Eperythrozoon wenyonii in cow from Fengdu with universal primer of haemotrophic bacteria.The result showed that the sequence length of Eperythrozoon wenyonii was 1 454 bp,GenBank accession number was FJ375309（Fengdu strain）.The determinated sequence was most similar to that of AY946266（Wuhan strain ）（with the homology of 99.7%）.Phylogenetic analysis indicated that it was more similar to those of Mycoplasma spp.in Mycoplasmataceae（homology level between 60.7%and 76.2%）,but was farther from Rickettsia spp.in Rickettsiaceae and Anaplasma spp.in Anaplasmataceae（homology level between 51. 4%and 56.4%）.These data indicated that E.xvenyoni seems to belong to Mycoplasmataceae.Eperythrozoon,but not Rickettsials,Anaplasmataceae.Genetic relationship analysis of Eperythrozoon wenyonii isolates indicated that there was any apparente tendency of geographical grouping of Eperythrozoon wenyonii in the world.

关 键 词：牛 温氏附红细胞体 16S RRNA 序列分析 

分 类 号：S823[农业科学—畜牧学]