山羊支原体山羊肺炎亚种甘肃株的分离及鉴定  

Isolation and identification of Mycoplasma capricolum subsp.capripneumoniae in Gansu provicne

作　　者：郭晗[1] 储岳峰[1] 赵萍[1] 高鹏程[1] 贺英[1] 樊祜卿[1] 冯小明[1] 逯忠新[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部草食动物疫病重点开放实验室,农业部畜禽病毒学重点开放实验室,甘肃兰州730046

出　　处：《中国兽医学报》2011年第3期352-356,共5页Chinese Journal of Veterinary Science

基　　金：甘肃省农业生物技术专项(GNSW-2010-09);甘肃省科技支撑计划项目(1011NKCA054);国家科技基础性工作专项(2008YF210200)

摘　　要：2007年甘肃某地羊场山羊发生以呼吸困难、咳嗽和胸膜肺炎为特征的呼吸道传染病,经临床诊断为羊支原体性肺炎。用改良的Thiaucourt's培养基对病山羊的肺组织进行病原分离,成功分离到1株支原体M1601,经菌落形态观察、生化试验、PCR鉴定、以16S rRNA基因序列构建的分子进化树分析和动物致病性试验,证明该分离株属于山羊支原体山羊肺炎亚种。In 2007,an outbreak of respiratory diseases of goats from a farm in Gansu province was diagnosed as goat mycoplasmal pneumonia based on clinical signs,which characterized by dyspnoea,cough and pleuropneumonia.One strain of mycoplasma,named as M1601,was isolated successfully from lung tissues of sick goats by using the modified Thiaucourfs medium.The M1601 strain was subjected to biochemistry test,PCR,16S rDNA sequences comparison analysis and animal pathogenicity test.The results suggest that the strain of M1601 belongs to the Mycoplasma capricolum subsp.capripneumoniae（Mccp）.

关 键 词：山羊支原体山羊肺炎亚种 山羊传染性胸膜肺炎 PCR鉴定 16S RRNA 

分 类 号：S852.65[农业科学—基础兽医学]