芪竹方粗提物对MGC-803细胞VEGF、KDR／flk-1蛋白表达的影响  

Infuence of rough extraction of QiZhu decoction on VEGF＆ KDR/flk-1 protein expression in MGC-803 cell

作　　者：徐陆周[1] 单兆伟[1] 赵万洲 

机构地区：[1]南京中医药大学附属江苏省中医院消化内科,210029 [2]南京凯基生物科技发展有限公司肿瘤分子生物实验室,210012

出　　处：《国际中医中药杂志》2011年第3期214-216,共3页International Journal of Traditional Chinese Medicine

摘　　要：目的 探讨芪竹方粗提物对MGC-803细胞血管内皮生长因子（vEGF）及其血管内皮生长因子受体（KDR／flk-1）蛋白表达的影响。方法MGC-803细胞处理方法被分成空白对照、二甲基亚砜（DMSO）对照、芪竹方粗提物高（500gg／m1）、低浓度（250gg／ml）四组，采用流式细胞仪、westernbloting两种方法测定VEGF、KDR／flk-1蛋白的表达量。结果空白对照、DMSO对照、芪竹方粗提物高、低浓度四组在流式细胞术检测中VEGF表达量分别是120．0±10．8、116．8±14．7、95．0±12．5、108．4±13．5，与空白对照组比较，芪竹方粗提物高浓度组VEGF表达降低，具有显著性差异；KDR／flk-1分别是10．4±3．5、9．0±3．4、6．8±2.3、6．8±3．5，各组间差异无统计学意义。在westernbloting法检测中VEGF蛋白表达量灰度值分别是14．45±4．61、12．32±3．27、2．58±0．84、3．45±1．12，芪竹方粗提物高浓度、低浓度组均明显低于空白对照组，差异具有显著性；KDR／ilk-1蛋白表达量灰度值分别是3．87±1．05、3．55±1.32、3．62±1．01、3．73±0．88，各组间差异无统计学意义。结论苠竹方粗提物对MGC．803细胞VEGF表达有下调作用，对KDR／flk-1表达无明显影响。Objective To evaluate the influence Qizhu decoction on VEGF and its receptor KDR/flk-1 protein expression in MGC803 cell. Methods Four groups of MGC-803 Cells were established, and intervened with blank control, DMSO control, high close Qizhu decoction, and low dose Qizhu decoction respectively. VEGF and KDP/flk-1 protein were detected by flow cytometry and western bloting. Results The value of VEGF expression was 120.0±10.8, 116.8±14.7, 95.0± 12.5, and 108.4±13.5 respectively in each group. The difference between high dosage Qizhu decoction group and the bland control group was significant. Value of KDR/ilk-1 were 10.4±3.5, 9.0±3.4, 6.8±2.3, and 6.8±3.5 in each group respectively. There was no significant difference among these 4 groups. The grayscale values of VEGF expression was 14.45 ±4.61, 12.32± 3.27, 2.58±0.84, and 3.45±1.12 in each group respectively. There was significant difference between the QiZhu groups and the blank control group. Meanwhile grayscale values of KDR/ilk-1 expression were 3.87 ± 1.05, 3.55±1.32, 3.62±1.01, and 3.73±0.88 in each group respectively, showing no significant difference among 4 groups. Conclusion Rough extraction of QiZhu decoction down-regulated the protein expression of VEGF, but had no effects on the expression of KDR/ilk-1.

关 键 词：芪竹方 MGC-803 VEGF:KDR/flk-1 

分 类 号：R28[医药卫生—中药学]