生长抑素受体亚型2、5在肺腺癌细胞株中的表达以及奥曲肽对肺腺癌细胞分泌TGF-β_1的影响  

Expression of 2,5 subtypes of somatostatin receptor in A-549 human lung adenocarcinoma cell line and effect of octreotide on secretion of TGF-β_1

作　　者：刘擂[1] 李宝平[1] 

机构地区：[1]山西医科大学第二附属医院肿瘤生物治疗科,太原030001

出　　处：《中国医疗前沿》2011年第2期7-8,共2页China Healthcare Innovation

摘　　要：目的研究A-549肺腺癌细胞株生长抑素受体亚型2、5(SSTR-2、SSTR-5)的表达情况及奥曲肽对A-549肺腺癌细胞株分泌转化生长因子β1(TGF-β1)的影响。方法采用S-P免疫组织化学法检测A-549细胞株中SSTR-2、SSTR-5的表达情况;采用酶联免疫吸附实验的方法(ELIZA)检测奥曲肽对A-549细胞分泌TGF-β1的作用。结果在A-549肺腺癌细胞株中,SSTR-2、SSTR-5均呈阳性表达。高浓度奥曲肽可以抑制A-549细胞自分泌的TGF-β1,且呈时间-剂量依赖性。结论 SSTR-2、SSTR-5于A-549肺腺癌细胞皆有表达;奥曲肽可以抑制A-549细胞分泌TGF-β1。Objective To observe the expression of subtypes of somatostatin receptor（SSTR） and the secretion of transforming growth factor β1（TGF-β1） and to investigate the effect of octreotide on secretion of TGF-β1 in A-549 human lung adenocarcinoma cell line.Methods Immunohistochemical S-P method was employed to examine the expression of subtypes of somatostatin receptor（SSTR）.The effect of octreotide on TGF-β1 secretion was measured by enzymes linked immunosorbant assay（ELIZA）.Results SSTR-2 and SSTR-5 was detected in A-549 human lung adenocarcinoma cell line.secretion of TGF-β1 in A-549 human lung adenocarcinoma cell can be proved.Octreotide do inhibit the secretion of VEGF in a dose-dependent and time-dependent manner.Conclusion A-549 cells do express SSTR-2 and SSTR-5;octreotide is an inhibitor on secretion and production of TGF-β1 in A-549 cell line.

关 键 词：肺癌 A-549 生长抑素受体 奥曲肽 TGF-β1 

分 类 号：R734.2[医药卫生—肿瘤]