HPLC-ELSD法同时测定乳制品中5种水溶性糖的含量  

Determination of Soluble Sugar in Dairy Product by HPLC-ELSD

作　　者：闫正[1] 张玉[1] 李盈辰[1] 李小亭[2] 赵志磊[2] 

机构地区：[1]河北大学化学与环境科学学院,河北保定071002 [2]河北大学质量技术监督学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2011年第1期52-56,共5页Journal of Hebei University(Natural Science Edition)

基　　金：质检公益性行业专项课题资助(200810432)

摘　　要：建立了HPLC-ELSD同时检测乳制品中5种水溶性糖的分析方法.将奶粉样品用60～70℃水溶解后超声波提取,亚铁氰化钾溶液和乙酸锌溶液沉淀蛋白,C18固相萃取小柱净化,碳水化合物色谱柱分离,V(乙腈)∶V(水)=80∶20流动相洗脱,蒸发光散射检测器检测.结果表明:果糖、半乳糖、蔗糖、麦芽糖和乳糖在1～10mg/mL内呈现良好的线性关系,加标平均回收率为93.0%～108.9%,RSD为0.85%～2.7%.该方法简便快速,灵敏度高,且重复性好,可为奶粉及其他乳制品中水溶性糖的检测提供技术支持.A quantitative assay for the determination of sugar,such as fructose,galactose,sucrose,maltose and lactose was established by high performance liquid chromatography（HPLC）.Sugars were extracted from samples with water by ultrasonic oscillation and isolated by solid phase extraction with cartridge after protein precipitation by using K4Fe（CN）6 and Zn（CH3COO）2.The separation was performed in the carbohydrate column（4.6 mm×250 mm）,acetonitrile：water（80∶20）（V/V） as mobile phase,Evaporative Light Scattering detector was adopted.The results showed that the linear relation of fructose galactose sucrose maltose and lactose was well in the range of 1～10 mg/mL,the average recoveries in the 93.0%-108.9%,RSD in the range of 0.85%～2.7%.The method was accuracy,sensitivity and feasibility to analyze the residues of fructose,galactose,sucrose,maltose and lactose in dairy products.

关 键 词：高效液相色谱法 乳制品 可溶性糖 

分 类 号：O657.72[理学—分析化学]