滤膜法测定水中大肠菌群简便方法的研究  

STUDIES OF SIMPLE METHOD ABOUT THE MEMBRANE FILTRATION METHOD FOR COLIFORM ORGANISMS IN WATER

作　　者：张淑兰[1] 韩秀媛[1] 路金爽[1] 

机构地区：[1]天津市卫生防病中心

出　　处：《中国微生态学杂志》1999年第4期231-232,共2页Chinese Journal of Microecology

摘　　要：本研究方法为ＩＳＯ９３０８－１规定的滤膜法测定水中大肠菌群的方法，该方法操作简便只需分离和证实两步实验即可得出结果；分离培养基上的阴阳性菌落颜色分明，易于分辩；所用培养基不含致癌物质。本研究方法的准确度高，回收率的均值为９４７％，批内变异系数小于１０％，检出限为５００ｍｌ水样可检出１个ＣＦＵ，检出率是传统方法的３１倍。本研究还探讨了分离培养基的ｐＨ值对菌落颜色的影响。本研究内容经查新在国内尚无报道。This method is the ISO 9308-1, the Membrane Filtration Method for Coliform Organisms in Water. It's easy to operate. Two stepsisolation and confirmation are only needed to get the result. On the isolation media, the color of the negative colony and that of positive one are easy to distinguish. The media used in the method doesn't contain the carcinogen. This method has the high accuracy. The average figure of the recovery ratio is 94.7%. CV is below 10%. The limit of detection is one CFU out of 500ml water. The detection ratio of this method is 3.1 times as much as the traditional method. In this method, the effect of pH of the isolation media on the colonies color is also studied. There is no other reprot about this area in China.

关 键 词：水 滤膜法 大肠菌群 国际标准 

分 类 号：R123.1[医药卫生—环境卫生学] R117[医药卫生—公共卫生与预防医学]