抗人胃癌单链抗体的分离纯化及其活性测定  

PURIFICATION AND ACTIVITY ASSAY OF SCFV ANTIBODY OF ANTI HUMAN STOMACH CANCER

作　　者：张淑梅[1] 李晶[1] 赵晓祥[1] 张云湖[1] 李荣萍[1] 杨志兴[1] 

机构地区：[1]黑龙江省科学院应用微生物研究所

出　　处：《生物技术》1999年第4期9-11,共3页Biotechnology

基　　金：黑龙江省科委攻关项目

摘　　要：用已经构建的工程菌高效表达抗人胃癌单链抗体，并使用ＳｅｐｈａｄｅｘＧ－１００柱层板分离纯化抗体蛋白。同时用免疫竞争抑制实验。e have expressed efficiently ScFv antibody of antihuman stomach cancer in constructed engineering strains. ScFv antibody were purified with SephadexG-100 gel-filtration chromatography, its binding activities against the stomach carcinoma cells were detected by immunological competing inhibition method.

关 键 词：胃癌 单链抗体 分离纯化 免疫竞争抑制 

分 类 号：R735.2[医药卫生—肿瘤]