大黄素和大黄酸的体外遗传毒性评价  

Evaluation of in vitro genotoxicity of emodin and rhein

作　　者：朱钦翥[1] 陈维[1] 张立实[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生学教研室,四川成都610041

出　　处：《癌变．畸变．突变》2011年第1期65-67,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家高技术研究发展计划(863计划)项目(2010AA023001)

摘　　要：目的:评价大黄素和大黄酸的体外遗传毒性。方法:使用不同浓度的大黄素和大黄酸(均分别为20、40、80、120μg/ml)处理人的类淋巴母细胞WTK1后,进行彗星实验、体外微核试验和TK基因突变试验。并设溶剂对照组和甲基甲烷磺酸(mthylmethane sulfonate,MMS)阳性对照组。结果:大黄素80和120μg/ml剂量组TK基因突变频率、细胞拖尾率及平均尾长均增高(P<0.05);大黄酸120μg/ml剂量组TK位点总突变频率增高(P<0.05)。结论:在本实验条件下,大黄素和大黄酸均表现出弱致突变作用。OBJECTIVE：To evaluate in vitro genotoxicity of emodin and rhein.METHODS：The WTK1 cells were treated by emodin and rhein of different concentrations（20,40,80,120μg/ml） and then the comet assay,in vitro micronucleus test and TK gene mutation assay were conducted simultaneously.The solvent control and positive control （mthylmethane sulfonate,MMS） groups were included in the study.RESULTS：Positive results were found at the concentrations of 120μg/ml and 80μg/ml of emodin in comet assay and tk gene mutation analysis.High dose of rhein （120μg/ml） also showed weak mutagenicity in TK gene mutation analysis.CONCLUSION：Under the present experimental conditions,emodin and rhein showed weak mutagenicity.

关 键 词：大黄素 大黄酸 遗传毒性 彗星试验 体外微核试验 TK基因突变试验 

分 类 号：R151.3[医药卫生—营养与食品卫生学]