rshCD_(40)L、IFN-γ对食管癌Eca109、Eca9706、TE13细胞增殖和凋亡的影响  

Expression of CD_(40) and growth-inhibitory activity of CD_(40) ligand in esophageal squamous cell carcinoma in virto

作　　者：吴广洲[1] 沈振亚[1] 刘国锋 张爱娟 吴雨岗[3] 

机构地区：[1]苏州大学附属第一人民医院,江苏苏州215006 [2]盐城市第一人民医院 [3]苏州大学附属第三人民医院

出　　处：《山东医药》2011年第6期23-25,共3页Shandong Medical Journal

摘　　要：目的观察可溶性重组人CD40L(rshCD40L)、IFN-γ对食管癌Eca109、Eca 9706、TE13细胞增殖和凋亡的影响。方法取正常培养的食管鳞癌细胞株Eca109、Eca 9706、TE13,分别用PBS、100 U/ml IFN-γ、100 ng/ml rsh-CD40L、100 U/ml IFN-γ+100 ng/ml rshCD40L培养,分别为A、B、C、D组。用MTT法测算各组细胞增殖抑制率,用TUNEL法检测细胞凋亡率。结果 C组Eca109、Eca9706、TE13细胞增殖抑制率分别为40.6%±4.2%、31.5%±5.7%、44.6%±6.7%,明显高于A、B组(P均<0.05);D组分别为56.7%±4.9%、41.2%±6.2%、51.6%±5.2%,均高于C组(P均<0.05)。C组Eca109、Eca9706、TE13细胞凋亡率分别为33.6%±3.7%、30.5%±2.8%和37.6%±4.9%,明显高于A、B组(P均<0.05);D组分别为43.7%±4.7%、34.2%±5.1%、41.5%±5.7%,均高于C组(P均<0.05)。结论 rshCD40L能促进食管癌Eca109、Eca9706、TE13细胞凋亡,并抑制其增殖。IFN-γ可增强这一作用。Objective To explore the expression of CD40 on esophageal squamous cell lines,and evaluated the effect of recombinant soluble human CD40L（rshCD40L） on esophageal cell lines.Methods The expression of CD40 on esophageal cell lines was examined by flow cytometry.The growth-inhibitory activity of rshCD40L on esophageal cancer cell was examined by MTT assay and the proportion of apoptotic tumor cells was examined in the TUNEL assay.Results The expression of CD40 was elevated in 3 esophageal cell lines examined by flow cytometry.CD40 expression could be induced by interferon-γ（IFN-γ）.rshCD40L inhibited the proliferation of theCD40＋ esophageal cancer cell lines significantly.The inhibition could also be enhanced by IFN-γ in Eca109、Eca9706 and TE13 lines.In addition,rshCD40L induced apoptosis of the CD40＋ esophageal cancer cell lines.Conclusions The expression of CD40 is involved in the carcinogenesis and progression of esophageal squamous cell carcinoma.CD40 present in esophageal cancer,and rshCD40L may be of clinical use to inhibit human esophageal cancer growth.

关 键 词：食管肿瘤 食管癌 CD40 rshCD40L 干扰素-Γ 细胞增殖 细胞凋亡 

分 类 号：R735.1[医药卫生—肿瘤]