Multi-drug Resistance and Characteristic of Integrons in Shigella spp.Isolated from China  

作　　者：ZHU JingYuan DUAN GuangCai YANG HaiYan FAN QingTang XI YuanLin 

机构地区：[1]Department of Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan China [2]Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, Henan China [3]Henan University of Science & Technology, Luoyang 471003, Henan, China [4]The Key Laboratory of Molecular Medicine in Henan Province, Zhengzhou 450052, Henan, China

出　　处：《Biomedical and Environmental Sciences》2011年第1期56-61,共6页生物医学与环境科学（英文版）

基　　金：supported by the Research Fund from Ministry of Health of the People’s Republic of China, WKJ2007‐2‐024

摘　　要：Objective To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigella spp. Methods Ninety Shigella strains （83 S. flexneri and 7 S. sonnei） were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them. Results High prevalence of multi‐drug resistance （95.6%） was identified. Of the isolates 79 （87.8%） carried integrase genes of class 1 integron （3.3%）, class 2 integron （10.0%） or both （74.4%）. No intI3 was detected in the tested isolates. The prevalence of intI2 was significantly higher in isolates with multi‐drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics （P0.05）. Gene cassettes dfrA17‐aadA5, dfrA12‐orfF‐aadA2 of class 1 integron and dfrA1‐sat1‐aadA1 of class 2 integron were identified. Conclusion The class 2 integron may play a role in the emergence of multi‐drug resistance in Shigella spp.Objective To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigella spp. Methods Ninety Shigella strains （83 S. flexneri and 7 S. sonnei） were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them. Results High prevalence of multi‐drug resistance （95.6%） was identified. Of the isolates 79 （87.8%） carried integrase genes of class 1 integron （3.3%）, class 2 integron （10.0%） or both （74.4%）. No intI3 was detected in the tested isolates. The prevalence of intI2 was significantly higher in isolates with multi‐drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics （P0.05）. Gene cassettes dfrA17‐aadA5, dfrA12‐orfF‐aadA2 of class 1 integron and dfrA1‐sat1‐aadA1 of class 2 integron were identified. Conclusion The class 2 integron may play a role in the emergence of multi‐drug resistance in Shigella spp.

关 键 词：Resistance Shigella flexneri INTEGRON Gene cassettes 

分 类 号：S945.12[农业科学—水产养殖] TQ462.8[农业科学—水产科学]