两个雌核发育白鲢群体同工酶分析及遗传标记的确定  

ISOZYME ANALYSIS AND PRELIMINARY CONFIRMATION OF THE GENETIC MARKERS IN TWO ARTIFICIAL GYNOGENETIC POPULATIONS OF SILVER CARP, HYPOPHTHALMICHTHYS MOLITRIX

作　　者：杨书婷[1] 桂建芳[1] 

机构地区：[1]中国科学院水生生物研究所

出　　处：《水生生物学报》1999年第3期264-268,共5页Acta Hydrobiologica Sinica

摘　　要：取源于武汉两个不同渔场两尾白鲢的卵子，经紫外照射遗传物质失活的鲤鱼精子刺激雌核发育和热休克诱导第二极体保留的基因组操作技术，获得了两个不同的人工雌核发育白鲢群体。采用聚丙烯酰胺垂直板电泳技术，分析了这两个不同人工雌核发育白鲢群体（分别称为Hy－G1和Hy－G2）内不同个体的肝脏、肌肉组织以及红细胞中乳酸脱氢酶（LDH）、苹果酸脱氢酶（MDH）、酯酶（EST）、超氧化物歧化酶（SOD）等几种同工酶的表达谱式，并与普通繁殖的同龄白鲢进行了比较。结果表明，各个雌核发育白鲢群体内不同个体间的酶谱表现出很大程度的一致性，具较高的纯合度，而两个不同雌核发育群体Hy－G1和Hy－G2之间表现有明显差异。特别是肝脏和肌肉组织迁移率较快的酯酶谱带等以其稳定的差异，建议作为区分这两个人工雌核发育白鲢群体的生化遗传标记。Two artificial gynogenetic populations were obtained from different broodstocks of silver carp, Hypophthalmichthys molitrix,originated from two fishery farms in Wuhan. The eggs of each broodstock were inseminated with UV-irradiated sperm of red common carp (Cyprinus carpio) and heat shock was performed through 39℃ for 1 min at 4 min after insemination for inducing diploid by inhibiting second polar body extrusion. Four isozyme zymograms, including esterase (EST), lactate dehydrogenase (LDH), malate dehydrogenase (MDH) and superoxide dismutase (SOD),were analysed by polyacrylamide gradient gel electrophoresis in liver, muscle and red blood cells from the two artificial population silver carps. In comparison with the popular hatchery silver carps, there were more similar isozyme zymograms among the individuals of artificial gynogenetic silver carp, and therefore, there existed higher homogeneity because of gynogenesis. Additionally, obvious difference between the gynogenetic populations and the hatchery populations was found from the isozyme zymograms. Some bands specific to the different gynogenetic populations wereobserved in EST isozyme zymograms in liver and muscle. Therefore, the specific bands would be suggested as the genetic markers of the two artificial gynogenetic populations of silver carp.

关 键 词：同工酶 人工雌核发育 遗传标记 白鲢 

分 类 号：S965.113[农业科学—水产养殖]