肝硬化患者血清苹果酸脱氢酶的活性检测  

Study on activity of serum malate dehydrogenase in patients with liver cirrhosis

作　　者：陈芊伊[1] 吕星[1] 张志杰[1] 代洪[1] 

机构地区：[1]湖南师范大学医学院医学检验系,湖南长沙410006

出　　处：《临床和实验医学杂志》2011年第5期347-349,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨肝硬化患者血清苹果酸脱氢酶(MDH)的活性变化及其临床意义。方法 95例血清分为肝硬化组48例、肝炎组20例和正常对照组27例。采用终点法测定肝硬化患者血清MDH活性,计算MDH对诊断肝硬化的阳性率、灵敏度和特异性,并分析血清MDH与白蛋白(ALB)、丙氨酸氨基转移酶(ALT)、总胆红素(TB)的相关性。结果肝硬化组血清MDH活性为(72.60±7.90)U/L,肝炎组患者血清MDH活性(77.47±7.03)U/L,正常组血清MDH活性为(38.58±3.54)U/L。肝硬化组、肝炎组均显著高于正常对照组,有显著性差异(P<0.01),但肝硬化与肝炎组间无明显差异(P>0.05)。MDH活性与ALB、ALT、TB均无相关性。阳性判断以MDH>50 U/L为标准,其敏感度为100%,特异性为96.3%。结论肝硬化患者有MDH活性增高,可为辅助诊断肝硬化提供有价值的信息。Objective To evaluate the activity and clinical significance of serum malate dehydrogenase（MDH） in patients with liver cirrhosis.Methods 95 cases were divided into 48 cases of liver cirrhosis,20 cases of hepatitis and 27 cases of normal control group.The serum activity of MDH was detected by endpoint method.The positive rate,negative rate,sensitivity and specificity of serum MDH in diagnosis of patients with liver cirrhosis were evaluated.The correlation among MDH and ALB,ALT and TB was studied and analyzed.Results The activity of serum MDH in cirrhosis group was 72.60±7.90 U/L,hepatitis group was 77.47±7.03 U/L,and it was 38.58±3.54 U/L in normal control group.Cirrhosis,hepatitis group was significantly higher than the control group,the difference was significant（P〈0.001）;but no significant difference between Cirrhosis and hepatitis groups（P〉0.05）.The activity of serum MDH had no correlation with serum levels of ALB,ALT and TB.Positive judgments to MDH 50 U / L as the standard,the sensitivity was 100% and specificity of 96.3%.Conclusion The serum activity of MDH in patients with liver cirrhosis was increased,and the activity of serum MDH can be served as a referential index in diagnosis and differential diagnosis of liver cirrhosis.

关 键 词：肝硬化 苹果酸脱氢酶 活性 

分 类 号：R575.2[医药卫生—消化系统]