S100A11在结肠癌的表达及其与临床特征的关系  

Expression and clinical features of S100A11 in colon carcinoma tissues

作　　者：李春成[1] 贺修胜[1] 王妍[1] 陈苏琼[1] 姚旭炯[1] 李丽[1] 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001

出　　处：《现代生物医学进展》2011年第2期338-340,331,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨S100A11在结肠癌和正常肠粘膜组织中的表达及其与患者临床特征的的关系。方法:采用RT-PCR、WesternBlotting技术,检测S100A11在24例结肠癌及正常肠粘膜中的表达,并分析S100A11与患者年龄,性别,临床病理分型之间的关系。结果:S100A11mRNA在结肠癌组织的表达量(0.944+0.032)高于正常肠粘膜组织中的表达量(0.828+0.079),两组比较差异有统计学意义(p<0.05)。S100A11蛋白在结肠癌组织中的表达量(0.951+0.02)高于在正常肠粘膜组织中的表达量(0.860+0.05),两组比较差异有统计学意义(p<0.05)。但与患者临床特征之间比较差异无统计学意义(p>0.05)。结论:S100A11在结肠癌组织中表达量高于正常肠粘膜组织,提示其与结肠癌的发生和发展有关.是判断结肠癌生物学行为的有价值的参考指标。Objective：To study the expression of S100A11 in normal and carcinoma colon tissues and the correlation between the result and clinical feature.Methods：The expression of S100A11 in 24 colon carcinoma and normal colon tissue were examined by RT-PCR and Western Blotting.The relationship between S100A11 and clinical parameters including patient＇s age,sex,differention de-gree were analyzed.Result：Expression intensity value of S100A11 mRNA was 0.944＋0.032 in human colon cancer tissues,Which of hu-man normal colon mucous membrane Tissue was 0.828＋0.079（p〈0.05）.Expression intensity value of S100A11 protein was 0.951＋0.02 in human colon cancer tissue,Which of human normal colon mucous membrane tissue was 0.860＋0.05（p〈0.05）.Results suggest the expression of S100A11 in colon cancer tissues was higher than that in normal colon tissues.Conclusion：Expression of S100A11 in colon cancer tissues was higher than that in normal colon tissue.Which indicates that S100A11 would play a significant role in the tumorrigenesis and development of colon cancer,therefore,S100A11could be regarded as a valuable prediction index that aims to judge biological behavior of colon cancer.

关 键 词：S100A11 结肠癌 RT-PCR WESTERN BLOTTING 

分 类 号：R735.35[医药卫生—肿瘤]