采用巢式PCR-RFLP检测胰液中突变的Kras基因  

Detection of K-ras Gene Mutant in Pancreatic Juice by Nested PCR-RFLP

作　　者：刘华平[1] 李方跃[1] 梁伟[2] 徐阿曼[1] 肖尚喜[1] 

机构地区：[1]安徽医科大学第一附属医院,合肥230022 [2]安徽省立医院普外科,合肥230001

出　　处：《中国生物制品学杂志》1999年第3期162-164,共3页Chinese Journal of Biologicals

基　　金：安徽省卫生厅医学科研基金资助!编号 94-04

摘　　要：采用多聚酶链 限制性片段长度多态性检测技术 ,对临床上可疑的胰腺炎和胰腺癌患者 ,经收集胰液进行K ras基因第 12位密码子突变检测 ,以诊断或鉴别诊断胰腺癌。结果在 2 2份胰液标本中 ,11例胰腺炎均无K ras基因突变 ;在 11例胰腺癌病例中有 9例 (81 2 % )含KIn order to identify pancreatic cancer cases,the mutant at the 12th codon of K ras gene in the pancreatic juice from the doubtful patients with pancreatitis and pancreatic cancer were detected by nested PCR RFLP.No K ras gene mutant was detected out in the 11 specimens from the patients with pancreatitis,however,it was detected out in 9(81.2%) of the 11 specimens from those with pancreatic cancer.

关 键 词：PCR-RFLP 胰腺癌 基因突变 K-RAS基因 

分 类 号：R735.902[医药卫生—肿瘤]