急性动脉粥样硬化性脑梗死患者外周血单核细胞4-1BB配体表达水平的变化  

Change of 4-1BB ligand expression of peripheral blood mononuclear cells in patients with atherosclerotic acute cerebral infarction

作　　者：刘广志[1] 苗丹[2] 高旭光[1] 

机构地区：[1]北京大学人民医院神经内科,北京100044 [2]青岛市立医院神经内科

出　　处：《临床神经病学杂志》2011年第1期8-10,共3页Journal of Clinical Neurology

摘　　要：目的研究急性动脉粥样硬化性脑梗死(AC I)患者外周血单核细胞(PBMC)4-1BB配体(4-1BBL)表达水平的变化。方法通过流式细胞术和酶联免疫吸附分析(ELISA)测定20例ACI患者PBMC4-1BBL的表达和血浆可溶性4-1BBL(s4-1BBL)的浓度,并与19例动脉粥样硬化患者及23名健康对照者进行比较,并对部分患者做了连续观察。结果与正常对照组CD14+单核细胞上4-1BBL的阳性细胞率和平均荧光强度(MFI)[(4.6±2.4)%,(4.1±2.7)]和血浆s4-1BBL(75 pg/ml)比较,ACI组[(7.9±4.1)%,(7.0±4.0),133.2 pg/ml]显著升高,差异均有统计学意义(均P<0.05),与动脉粥样硬化组阳性细胞率[(6.3±5.0)%]和MFI(5.2±2.3)和血浆s4-1BBL(84 pg/ml)比较差异均无统计学意义;在ACI恢复期4-1BBL的阳性细胞率和MFI分别为(7.6±5.4)%和(5.6±2.1),血浆s4-1BBL水平为113.2 pg/ml,与急性期相比呈下降趋势,但差异无统计学意义。结论 ACI患者4-1BBL的表达上调是ACI发病早期即被诱导产生的一个异常免疫应答,在动脉粥样硬化所致缺血性脑卒中的发病过程中可能发挥着重要的作用。Objective To investigate the change of 4-1BB ligand（4-1BBL） expression of peripheral blood mononuclear cells in patients with acute atherosclerotic cerebral infarction（ACI）.Methods Flow cytometry and enzyme-linked immunosorbent assay（ELISA） were used to detecte 4-1BBL expression in peripheral blood mononuclear cells and plasma solube 4-1BBL（s4-1BBL） protein level in 20 ACI patients（ACI） as compared to nineteen atherosclerosis patients and twenty-three normal controls.Serial studies were performed on some of these ACI patients.Results Compared with the expression of 4-1BBL positive cell rate and mean fluorescence intensity（MFI） on CD14＋monocytes and plasma s4-1BBL levels in normal control group [（4.6±2.4）%,（4.1±2.7）,75 pg/ml],the ACI group [（7.9±4.1）%,（7.0±4.0）,133.2 pg/ml] increased significantly,and all the differences were significant（all P0.05）;while no significant differences were found when comparing to the expression of 4-1BBL positive cell rate [（6.3±5.0）%] and MFI on CD14＋ monocytes（5.2±2.3） and plasma s4-1BBL levels（84 pg/ml） in atherosclerosis group.Compared with acute stage,the expression of 4-1BBL positive cell rate [（7.6±5.4）%] and MFI on CD14＋ monocytes（5.6±2.1） and plasma s4-1BBL levels（113.2 pg/ml） showed a tendency of decrease in the recovery stage of ACI,but there was no significant difference.Conclusion The 4-1BBL expression is upregulation in ACI patients,as an abnormal immune response that may have been induced during early stages of ACI,may play an important role in the process of atherosclerosis-related ischemic stroke.

关 键 词：急性脑梗死 4-1BB配体 外周血 动脉粥样硬化 

分 类 号：R743.3[医药卫生—神经病学与精神病学]