Senegenin promotes in vitro proliferation of human neural progenitor cells  

作　　者：Shi, Fang Liang, Zhigang Guo, Zixuan Li, Ran Yu, Fen Zhang, Zhanjun Wang, Xuan Wang, Xiaomin 

机构地区：[1]Capital Med Univ, Dept Physiol, Key Lab Neurodegenerat Disorders, Minist Educ, Beijing 100069, Peoples R China [2]Beijing Normal Univ, State Key Lab Cognit Neurosci & Learning, Beijing 100875, Peoples R China

出　　处：《Neural Regeneration Research》2011年第3期171-176,共6页中国神经再生研究（英文版）

基　　金：the National Basic Research Program of China-973 Project,No. 2006CB500700;the National Natural Science Foundation of China,No. 30500255;the Natural Science Foundation of Beijing,No. 5102010

摘　　要：Senegenin, an effective component of Polygala tenuifolia root extract, promotes proliferation and differentiation of neural progenitor cells in the hippocampus. However, the effects of senegenin on mesencephalon-derived neural progenitor cells remain poorly understood. Cells from a ventral mesencephalon neural progenitor cell line （ReNcell VM） were utilized as models for pharmaceutical screening. The effects of various senegenin concentrations on cell proliferation were analyzed, demonstrating that high senegenin concentrations （5, 10, 50, and 100 μmol/L）, particularly 50 μmol/L, significantly promoted proliferation of ReNcell VM cells. In the mitogen-activated protein kinase signal transduction pathway, senegenin significantly increased phosphorylation levels of extracellular signal-regulated kinases. Moreover, cell proliferation was suppressed by extracellular signal-regulated kinase inhibitors. Results suggested that senegenin contributed to in vitro proliferation of human neural progenitor cells by upregulating phosphorylation of extracellular signal-regulated kinase.Senegenin, an effective component of Polygala tenuifolia root extract, promotes proliferation and differentiation of neural progenitor cells in the hippocampus. However, the effects of senegenin on mesencephalon-derived neural progenitor cells remain poorly understood. Cells from a ventral mesencephalon neural progenitor cell line （ReNcell VM） were utilized as models for pharmaceutical screening. The effects of various senegenin concentrations on cell proliferation were analyzed, demonstrating that high senegenin concentrations （5, 10, 50, and 100 μmol/L）, particularly 50 μmol/L, significantly promoted proliferation of ReNcell VM cells. In the mitogen-activated protein kinase signal transduction pathway, senegenin significantly increased phosphorylation levels of extracellular signal-regulated kinases. Moreover, cell proliferation was suppressed by extracellular signal-regulated kinase inhibitors. Results suggested that senegenin contributed to in vitro proliferation of human neural progenitor cells by upregulating phosphorylation of extracellular signal-regulated kinase.

关 键 词：SENEGENIN neural progenitor cells cell proliferation mitogen-activated protein kinases 

分 类 号：Q421[生物学—神经生物学] Q253[生物学—生理学]