胃癌手术前后外周血中CEA mRNA的检测及临床意义  

Detection of CEA mRNA perioperatively in the peripheral blood of patients with gastric cancer and its clinical significance

作　　者：吕成余[1] 沈振伟[1] 张蕴春[1] 陈兴国[1] 杨士勇[1] 时开网[1] 

机构地区：[1]南京医科大学附属南京第一医院普外科,南京210006

出　　处：《外科理论与实践》2011年第1期63-67,共5页Journal of Surgery Concepts & Practice

基　　金：南京医科大学科技发展基金重点项目(07NMUZ042)

摘　　要：目的:探讨胃癌病人手术前后外周血中微转移标志物癌胚抗原(CEA)mRNA表达及临床意义。方法:采用巢式实时PCR技术检测50例胃癌病人手术前后的外周血中CEA mRNA的表达情况,并选择20名健康志愿者作为阴性对照组,选择10例新鲜胃癌组织作为阳性对照组。结果:胃癌病人术前外周血中CEA mRNA的表达阳性率为48.00%(24/50),术后阳性率为60.0%(30/50),差异无统计学意义(P>0.05);其阳性表达率与肿瘤的组织学类型及临床分期间具有相关性(P<0.05),而与病人性别、年龄及有无淋巴结转移间无统计学相关性(P>0.05);20名健康志愿者外周血中均无CEA mRNA阳性表达;而在10例新鲜胃癌组织中,均显示阳性表达。结论:CEA mRNA的表达可能与肿瘤微转移相关,采用巢式RT-PCR方法检测外周血中CEA mRNA的表达,可能是一种较理想的预测方法。手术对微转移影响尽管存在,但在临床上尚无统计学意义。Objective To investigate the expression of CEA mRNA perioperatively in the peripheral blood of patients with gastric cancer and its clinical significance.Methods The expression of CEA mRNA was detected in 10 fresh gastric cancer tissue specimens,in the peripheral blood of fifty patients with gastric cancer both pre-and post-operatively,and also in the peripheral blood of 20 healthy persons by nested-reverse transcription polymerase chain reaction（RT-PCR）.Results The positive rate for CEA mRNA in the peripheral blood of preoperative patients with gastric cancer was 48.0%,The positive rate for CEA mRNA in the peripheral blood of postoperative patients with gastric cancer was 60.0%,there was no significant statistical correlation（P0.05）.The positive rate for CEA mRNA was correlated with the pathologic differentiation and clinical states（P0.05）.But it had no significant correlation with the gender,age or the status of lymph node metastasis（P0.05）.The expression of CEA mRNA was positive in all gastric cancer tissue specimens,but negative in the peripheral blood of healthy people.Conclusions It is perhaps an ideal method to detect the micrometastasis of gastric cancer through the evaluation of expression of CEA mRNA in the peripheral blood by nested reverse transcription PCR（RT-PCR）.It may play an important role in the evaluation of the prognosis and the necessity of adopting advanced therapy among the gastric cancer patients.Surgery operation does influence the status of micrometastasis of gastric cancer patients,but no significant correlation has been found clinically.

关 键 词：巢式逆转录聚合酶链式反应 胃肿瘤 外科手术 微转移 

分 类 号：R735.2[医药卫生—肿瘤]