NF-κB特异性抑制剂PDTC对人肝门部胆管癌细胞增殖及SMYD3表达的影响  

Effect of Proliferation and SMYD3 Expression of Human Cholangiocarcinoma Cell Line QBC939 Induced by NF-κB Inhibitor Pyrrolidine Dithiocarbamate

作　　者：刘勇刚[1] 李志花[1] 陈汝福[2] 郭宁[2] 程帝[1] 廖巧芳[1] 

机构地区：[1]中山大学孙逸仙纪念医院肿瘤科,广东广州510120 [2]中山大学孙逸仙纪念医院肝胆外科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2011年第1期22-25,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30872485)

摘　　要：【目的】研究核转录因子-κB(NF-κB)特异性抑制剂吡咯烷二硫代氨基甲盐酸对肝门部胆管癌QBC939细胞增殖及组蛋白甲基转移酶表达的影响。【方法】不同浓度的PDTC作用于QBC939细胞不同时间后,CCK-8测定其对QBC939细胞增殖的抑制率,流式细胞仪观察QBC939细胞的周期变化情况,RT-PCR检测PDTC作用下QBC939细胞中SMYD3 mRNA的表达情况,Western blot法检测QBC939细胞内SMYD3蛋白的表达。【结果】经PDTC处理的实验组,肿瘤细胞增殖活性明显受到抑制(P<0.05),呈时间和剂量依赖性;与对照组相比,PDTC作用后QBC939细胞G0/G1期细胞比例上升(P<0.05),并同时伴有癌细胞内SMYD3 mRNA和蛋白表达的降低(P<0.01),且呈明显的剂量依赖关系。【结论】PDTC具有显著抑制肝门部胆管癌QBC939细胞生长的作用,其机制可能与细胞增殖抑制、周期阻滞、以及NF-κB、SMYD3表达抑制有关。【Objective】To explore the effect of pyrrolidine dithiocarbamate（PDTC） on proliferation of human cholangiocarcinoma cell line QBC939 and expression of SET and MYND domain-containing protein 3（SMYD3）.【Methods】 QBC939 cells were respectively treated in vitro with PDTC by different concentration.The inhibition rates of cell growth were assayed by CCK-8 method.Cell cycles were analyzed by flow cytometry（FCM）.The expression of SMYD3 was detected by RT-PCR and Western blot.【Restults】 The inhibition rates of QBC939 cell growth increased in a time-dependent and dose-dependent manner after treated with PDTC（P 0.05）.In contrast to the control,the percentage of cells in G0/ G1 phase increased significantly in PDTC group（P 0.05）,and the mRNA and protein expression of SMYD3 were downregulated（P 0.01）.【Conclusion】PDTC has the synergistic effects of anti-cholangiocarcinoma,which may be caused by inhibiting proliferation of QBC939,arresting its cell cycle and downregulating the expression of NF-κB and SMYD3.

关 键 词：胆管癌细胞QBC939 PDTC 核转录因子-KB 增殖 组蛋白甲基转移酶 

分 类 号：R735.8[医药卫生—肿瘤]