盐酸表阿霉素与牛血清白蛋白的相互作用和伏安分析  

Interaction of the Epirubicin Hydrochloride with Bovine Serum Albumin and Its Application

作　　者：程红芬[1] 张勇[1] 宋洁琼[1] 王增巧[1] 

机构地区：[1]山西大学化学化工学院,山西太原030006

出　　处：《电化学》2011年第1期80-83,共4页Journal of Electrochemistry

基　　金：国家自然科学基金项目(20375024);山西省青年科技基金项目(20021008)资助

摘　　要：应用线性扫描技术研究盐酸表阿霉素的电化学行为.结果表明,在pH 6.80的Tris-HCl缓冲溶液中,盐酸表阿霉素有一较灵敏的还原峰,Epc=-0.34 V(vs.SCE).加入牛血清白蛋白(BSA)后,盐酸表阿霉素的还原峰电流明显下降.据此,建立了BSA的电化学测定法.在最佳实验条件下,1.0×10-9～1.0×10-6 mol.L-1范围内,BSA浓度与盐酸表阿霉素的峰电流下降值△ip呈线性关系(R=0.9973),BSA检出限达8.05×10-10 mol.L-1.盐酸表阿霉素与BSA的结合比为1,结合常数β为3.04×106 L.mol-1.The linear sweep technique was used to study the electrochemical behavior of epirubicin hydrochloride.The results showed that there was a sensitive reduction peak at-0.34 V（vs.SCE） in pH 6.80 Tris-HCl buffer.When the BSA was added,the value of the reduction peak was reduced deeply.Accordingly,a new electrchemical determintation method of BSA was established.Under the optimum conditions,the decrease of the reduction peak currents of epirubicin hydrochloride were proportional to the concentrations of BSA in the range of 1.0×10-9～1.0×10-6 mol·L-1（R=0.9973）.The detection limit of the BSA was 8.05×10-10 mol·L-1.And the binding constant β of the epirubicin hydrochloride and BSA was 3.04×106 L·mol-1 with the ratio of 1.

关 键 词：盐酸表阿霉素 牛血清白蛋白(BSA) 玻碳电极 

分 类 号：R914[医药卫生—药物化学]