Oct4在多种人腺癌组织中表达水平的研究  

Research the expression of Oct4 in multiple adenocarcinoma

作　　者：张金龙[1] 王海娟[2] 路中[1] 刘健[2] 林晨[2] 钱海利[2] 李贵新[1] 

机构地区：[1]潍坊医学院,山东潍坊261053 [2]北京协和医学院肿瘤医院肿瘤研究所分子肿瘤学国家重点实验室,北京100021

出　　处：《中国医药导报》2011年第8期17-20,共4页China Medical Herald

基　　金：国家自然科学基金面上项目(30973447)

摘　　要：目的:研究5种人腺癌中Oct4的表达情况,为进一步探索其在肿瘤发生中的机制提供基础。方法:应用乳腺癌、胃癌、胰腺癌、肠癌、子宫内膜癌5种腺癌的组织芯片,通过免疫组织化学染色研究Oct4蛋白在组织中的表达情况,并与癌旁正常组织进行比较。结果:Oct4在5种腺癌肿瘤组织内均有表达,胃癌组织内Oct4的表达高于癌旁正常组织(P<0.05),其他腺癌组织中Oct4的表达与癌旁组织差异无统计学意义;在乳腺癌和肠癌中,无淋巴结转移患者的肿瘤组织中Oct4表达高于淋巴结转移患者的肿瘤组织(P<0.05),而在胃癌、胰腺癌及子宫内膜癌患者中未观察到这种差异;5种腺癌的肿瘤组织内,未发现Oct4的表达与临床分期有关。Oct4在癌旁正常组织中(子宫内膜癌除外)主要定位于细胞浆,而在腺癌组织中既表达于细胞核,也表达于细胞浆。结论:Oct4的表达可能是肿瘤发生过程中的早期事件,不同腺癌组织中Oct4的表达情况不完全相同,Oct4的核/浆表达模式可能与其在肿瘤发生中的作用有关。Objective： To study the expression of Oct4 in adenocarcinomas from 5 tissue origins and provide insights to the Oct4-related mechanism of carcinogensis. Methods： Immuno-histochemistry staining was adopted to study the expression of Oct4 protein in adenocareinoma tissue array containing adenocarcinoma and paratumoral samples of 5 tissue origins. Results： Oct4 was expressed in all 5 adenocarcinomas. Oct4 was significantly differently expressed in gastric-derived adenocarcinoma and paratumoral tissue （P〈0.05）, but not other origins. Breast and intestinal adenocarcinomas without lymph riode metastasis had stronger Oct4 expression than those with lymph node metastasis （P〈0.05）. However, there was no dif- ference of Oct4 level in gastric cancer, pancreatic cancer and endometrial cancer, no association of Oct4 expression with clinical stage was observed. Except for in endometrium tissue, Oct4 was mainly located in cytoplasm in normal tissue, but in nucleus and cytoplasm in tumor tissue. Conclusion： The expression of Oct4 may be an early event during tumorigenesis; Oct4 was expressed in different patterns in various tissues and their counterpart adenocarcinoma. The distribution of Oct4 in nuclear or cytoplasm may present its physiolozical or pathological function during tumorigenesis.

关 键 词：OCT4 腺癌 组织芯片 免疫组织化学 

分 类 号：R730.2[医药卫生—肿瘤]