HLA-DRB1、DQB1多态性与大肠癌化疗前后肝功能变化的关联  

Association between Polymorphism of HLA-DRB1、DQB1 and Injury of Liver Function from Colorectal Cancer Chemotherapy

作　　者：佟凤芝 侯艺 王娜 郭楠 赵新宇[2] 刘辉[2] 刘明开[3] 王婷婷[3] 

机构地区：[1]辽宁省大连市第五人民医院检验科,116021 [2]大连医科大学检验医学院,116044 [3]大连大学附属新华医院检验科,116021

出　　处：《中国医疗前沿》2011年第3期12-13,共2页China Healthcare Innovation

摘　　要：目的通过大肠癌患者在化疗前和化疗结束时的肝功能检验指标的变化以及其与HLA-DRB1、HLA-DQB1多态性的关联,以期找到大肠癌化疗副损伤的相关基因。方法选取100例大肠癌患者,采用多聚酶链反应-序列特异性引物(PCR-SSP)法检测其HLA-DRB1、HLA-DQB1的多态性。同时检测大肠癌患者化疗前和化疗结束时的肝功能检验指标,包括TBIL、DBIL、TP、ALB、ALT、AST、ALP、GGT。结果 HLA-DRB1*07有可能是化疗对ALT、GGT损伤的抵抗基因;HLA-DRB1*08有可能是化疗对ALT损伤的抵抗基因;HLA-DRB1*09有可能是化疗对ALB、ALT、GGT损伤的抵抗基因;HLA-DRB1*12有可能是化疗对ALB、ALT损伤的抵抗基因;HLA-DQB1*02有可能是化疗对ALT损伤的抵抗基因,同时HLA-DQB1*02也可能是化疗对AST损伤的敏感基因;HLA-DQB1*05有可能是化疗对ALB损伤的抵抗基因;HLA-DQB1*06有可能是化疗对ALT、GGT损伤的抵抗基因。结论 HLA可能是肿瘤化疗副损伤的标志性基因。Objective To explore the related genes of Subsidiary-damage in tumor chemotherapy by the change of liver function index of patients with colorectal cancer before and after chemotherapy and their correlation with the polymorphism of HLA-DRB1/DQB1.Methods Total 100 patients with colorectal cancer were selected.The DNA of every patient were extracted and the polymorphism of their HLA-DRB1,HLA-DQB1 was detected by PCR-SSP.Meanwhile,each index before and after chemotherapy were detected,including TBIL,DBIL,TP,ALB,ALT,AST,ALP,GGT.Results HLA-DRB1＊07 may be resistane gene of ALT,GGT injury;HLA-DRB1＊08 may be resistane gene of ALT injury;HLA-DRB1＊09 may be resistane gene of ALB、ALT,GGT injury;HLA-DRB1＊12 may be resistane gene of ALB、ALT injury;HLA-DQB1＊02 may be resistane gene of ALT injury and HLA-DQB1＊02 may be sensitive gene of AST injury;HLA-DQB1＊05 may be resistane gene of ALB injury;HLA-DQB1＊06 may be resistane gene of ALT、GGT injury.Conclusion HLA may be marker genes of secondary impair of tumour chems.

关 键 词：HLA-DRB1 DQB1 大肠癌化疗 副损伤 检验指标 

分 类 号：R730.45[医药卫生—肿瘤] R735.34[医药卫生—临床医学]