恒能量同步荧光光谱法测定食用油中多环芳烃  

Determination of Polycyclic Aromatic Hydrocarbons in Edible Oils by Constant-Energy Synchronous Fluorescence Spectrometry

作　　者：邱如斌 章汝平[2] 林水东[2] 李锦辉[2] 俞娇丽[2] 

机构地区：[1]闽西职业技术学院化工系 [2]龙岩学院化学与材料学院,福建省龙岩市364000

出　　处：《光谱实验室》2011年第2期777-781,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：福建省科技厅科技计划重点项目(2010Y0040);福建省科技厅资助省属高校项目(2007F5081);福建省自然科学基金资助项目(E0610001);福建省教育厅科学研究资助项目(JB08234)

摘　　要：用12%(w/V)的氢氧化钾-乙醇溶液皂化食用油的脂肪酸,以环己烷萃取皂化液中的多环芳烃,经浓缩,柱层析纯化并浓缩,取微量试样液稀释后,通过选择合适能量差,建立了同步荧光法测定食用油中多环芳烃含量的新方法,其线性范围为5—1000ng.mL-1,检出限在0.04—15.21ng.mL-1之间,平均加标回收率在81.94%—90.06%之间。该方法具有快速、简便、准确等特点。The fatty acid of edible oils was saponified by the solution of 12%（w/V） potassium hydroxide-ethanol.The polycyclic aromatic hydrocarbons of saponification solution were extracted by the cyclohexane.It was concentrated and decontaminated by column chromatography.A new method was described for the determination of polycyclic aromatic hydrocarbons in edible oils by synchronous fluorescence spectrometry with compatible constant-energy interval.The linear range was 5—1000ng·mL-1,with the detection limit of 0.04—15.21ng·mL-1.The recovery was between 81.94% and 90.06%.The method is quick and sensitive.

关 键 词：同步荧光光谱法 多环芳烃 皂化 净化 柱层析 

分 类 号：R155.5[医药卫生—营养与食品卫生学] O657.32[医药卫生—公共卫生与预防医学]