D_(101)型大孔树脂纯化茶皂素的研究  

Purification of tea sapon using D_(101) macroporous resin

作　　者：孙国金[1] 叶春林[2] 余勤[1] 

机构地区：[1]浙江中医药大学生物工程学院,杭州310053 [2]浙江科技学院生物与化学工程学院,杭州310023

出　　处：《食品科技》2011年第3期206-208,共3页Food Science and Technology

基　　金：浙江省科技计划项目(2008C32040)

摘　　要：研究了用D101型大孔树脂纯化茶皂素的工艺参数,当上样量为25mL、上样液浓度为30mg/mL、上样流速3mL/min时,吸附率达66.4%。用5倍树脂体积的85%乙醇洗脱,解吸率达83.6%,产品纯度92%。The study of optimal conditions of D101 macroporous resin was conducted for the purification of tea sapon. The optimal conditions for the purification of the macroporous resin were 30 mg/mL of tea saponin concentration of solution,3 mL/min of flow rate,25 mL of loaded amount,85% of ethanol concentration,5∶1 of the ratio of ethanol volume and bed volume. Under the conditions,the adsorption rate of tea saponin was 66.4%,the desorption rate of tea saponin was 83.6% and its purity reached 92%. The results showed that D101 macroporous resins was the suitable resin for purifying tea saponin.

关 键 词：茶皂素 大孔树脂 纯化 

分 类 号：TS272.2[农业科学—茶叶生产加工]