丹曲林对大鼠缺血再灌注心肌嘌呤核苷酸代谢的影响  

Effect of Dantrolene on purine nucleotide metabolism in myocardial ischemia-reperfusion in rats

作　　者：赵晓丽[1] 董嘉良[1] 王丽艳[1] 郭彦青[1] 曹颖[1] 于公元[1] 李海东[1] 

机构地区：[1]天津医科大学生物化学教研室,天津300070

出　　处：《中国现代医学杂志》2011年第2期181-184,188,共5页China Journal of Modern Medicine

摘　　要：目的探讨丹曲林对缺血再灌注心肌嘌呤核苷酸代谢的影响。方法 24只健康雄性Wistar大鼠(n=24)建立Langendorff离体心脏灌注模型,分为两组,对照组即缺血再灌注组和实验组即丹曲林处理组,应用高效液相色谱仪(HPLC)测定嘌呤核苷酸代谢产物腺苷,AMP,尿酸的含量变化,以揭示5’-核苷酸酶(5’-nu-cleotidase,5’-NT),腺苷脱氨酶(adenosine deaminase,ADA),黄嘌呤氧化酶(xanthine oxidase,XO)的活性。结果丹曲林处理组与缺血再灌注组相比,5’-核苷酸酶,腺苷脱氨酶,黄嘌呤氧化酶活性均明显增加(P<0.05)。结论丹曲林通过抑制Ca2+超载,降低Ca2+活化的钙蛋白酶对细胞膜骨架蛋白和结构蛋白的降解,以及增强膜磷脂的稳定性,维持细胞完整性,保存细胞内的各种代谢酶,对缺血再灌注心肌起到保护作用。【Objective】 To investigate the effect of Dantrolene on purine nucleotide metabolism in myocardial ischemia-reperfusion.【Methods】 Twenty-four male Wistar rats were randomly divided into two groups： control group and Dantrolene-treated group,Langendorff isolated heart model was used and the activity of three enzymes in purine nucleotide metabolism： 5＇-nucleotidase（5＇-NT）,adenosine deaminase（ADA）,and xanthine oxidase（XO） were measured by high-performance liquid chromatography（HPLC）.【Results】 The activity of 5＇-NT,ADA and XO in Dantrolene-treated group was significantly increased compared with the control group（P 0.05）.【Conclusion】 Dantrolene has a protective effect in myocardial ischemia-reperfusion by inhibiting the Ca2＋ overload,reducing Ca2＋ dependent activation of calpain,inhibiting the degradation of membrance skeleton proteins and structural proteins,increasing the stability of membrane phospholipids to maintain cell integrity and to keep metabolic enzymes within the cell.

关 键 词：丹曲林 钙蛋白酶 细胞骨架 核苷酸代谢 

分 类 号：R-332[医药卫生]