二烯丙基三硫对急性肺损伤小鼠血清细胞因子IL-18,IL-10的影响  

Effect of Diallyl Trisulfide on IL-18,IL-10 in mice with acute lung injury induced by Lipopolysaccharide

作　　者：康兴斌[1] 马凯峰[1] 张亚丽[2] 赵红林[3] 李春海[4] 

机构地区：[1]华北油田公司总医院心胸外科,河北任丘062552 [2]华北油田公司总医院普外科,河北任丘062552 [3]华北油田公司总医院肿瘤科,河北任丘062552 [4]河北北方学院附属第二医院心内科,河北张家口075000

出　　处：《中国现代医学杂志》2011年第2期185-188,共4页China Journal of Modern Medicine

摘　　要：目的探讨二烯丙基三硫(DATS)对急性肺损伤小鼠血清IL-18,IL-10的影响。方法 90只健康昆明小鼠,随机分为5组:①急性肺损伤组:腹腔注射大肠杆菌脂多糖(LPS);②预防组:腹腔注射DATS 7d后注射LPS;③治疗组:腹腔注射LPS后30min注射DATS;④DATS组:腹腔注射DATS 7d;⑤对照组:腹腔注射等量生理盐水。光镜观察12h点肺组织形态学改变。测定血清中IL-18及IL-10在2h和6h含量。结果 DATS预防组肺泡间渗出及炎性细胞浸润较ALI组明显减轻。其血清中IL-18含量均明显低于ALI组(P<0.01),IL-10含量均明显高于ALI组(P<0.01)。结论预防性应用DATS可减轻LPS诱导的ALI小鼠肺组织炎症反应。并能抑制LPS诱导的小鼠血清中炎性细胞因子IL-18的生成,而促进抗炎性细胞因子IL-10的生成。【Objectives】 To observe the effect of DATS on the inflammatory cytokines IL-18,IL-10 in serum of LPS-induced ALI mice.【Methods】 Ninety healthy Kun-ming mice were randomly divided into five groups： ALI group： mice were infused LPS intraperitoneally;DATS prevention group： mice were infused DATS intraperitoneally for 7 days and then received LPS injection;DATS treatment group： mice were infused LPS intraperitoneally and then received DATS injection 30 min later;DATS group： mice received DATS injection intraperitoneally for 7 days and without LPS injection;Control group： mice were received the same bulk of sodium chloride injection.The histopathology changes of lung tissues at 12 h were examined under light microscopy.The levels of IL-18 and IL-10 in the serum were detected with ELISA at 2 h and 6 h.【Results】 In DATS prevention group,the concentration of IL-18 in the serum decreased significantly while the concentration of IL-10 increased significantly at 2 h and 6 h（P 0.01 vs ALI group）.【Conclusions】 Pretreatment with DATS could alleviate the degree of pulmonary inflammation in LPS-induced ALI mice.Pretreatment with DATS could inhibit the production of IL-18 and increase the production of IL-10 in the serum in LPS-induced ALI mice.

关 键 词：急性肺损伤 二烯丙基三硫 细胞因子 IL-18 IL-10 

分 类 号：R-332[医药卫生] R563