裙带菜多糖对胃癌细胞BGC-823的影响  

Effect of Polysaccharides from Undaria Pinnatifida on Gastric Carcinoma BGC-823 Cells

作　　者：商晓辉[1] 商晓丽[2] 张勇[3] 赵连梅[4] 张志涛[5] 李艳辉[3] 

机构地区：[1]河北工程大学医学院生物教研室,河北邯郸056029 [2]邯郸市第一医院检验科 [3]河北工程大学医学院 [4]河北医科大学第四附属医院科研中心 [5]邯郸市传染病医院

出　　处：《肿瘤防治研究》2011年第2期134-136,140,共4页Cancer Research on Prevention and Treatment

基　　金：邯郸市科技局2008年指令性课题资助项目(0823108062-2)

摘　　要：目的探讨裙带菜多糖(polysaccharides from the Undaria Pinnatifida,PUP)对胃癌细胞的增殖抑制作用及其机制。方法 MTT法分析裙带菜多糖(25、50、100μg/ml)分别作用24、48、72h后,对BGC-823细胞增殖的抑制作用;用透射电子显微镜观察不同浓度(25、50、100μg/ml)裙带菜多糖作用24h、48h、72h后凋亡细胞的结构变化;不同浓度裙带菜多糖(25、50、100μg/ml)作用48h后,用流式细胞术分析BGC-823细胞的凋亡率,RT-PCR法检测survivin基因的表达水平。结果不同浓度的裙带菜多糖均可明显抑制胃癌细胞的增殖(P均<0.05),作用24h后的IC50为38.13μg/ml。不同浓度裙带菜多糖作用48h后,均可诱导BGC-823细胞凋亡,100μg/ml的裙带菜多糖可诱导BGC-823细胞凋亡,凋亡率达69.91%,导致BGC-823细胞发生凋亡特征性超微结构改变,并使BGC-823细胞survivin mRNA表达水平明显降低(P<0.01)。结论裙带菜多糖可抑制BGC-823细胞增殖,诱导肿瘤细胞凋亡,该作用与下调细胞survivin基因表达有关。Objective Effect of polysaccharides from the Undaria Pinnatifida on the inhibition of gastric carcinoma cell BGC-823 and its mechanism were studied.Methods After treatment with polysaccharides from the Undaria Pinnatifida at different concentrations（25,50,100 μg/ml）for 24 h,48 h,72 h,the inhibitory effect on proliferation of BGC-823 cells was analyzed by MTT method.After treatment with polysaccharides from the Undaria Pinnatifida under different concentrations（2.5,50,100 μg/ml） for 48 h,the cell structures were observed by transmission electron microscope.The cell apoptotic ratio of BGC-823 cells was measured with flow cytometry（FCM） and the expression of survivin mRNA was detected by RT-PCR.Results Polysaccharides from the Undaria Pinnatifida inhibited the proliferation of BGC-823 cells significantly in a concentration-depending manner（P0.05）.The IC50 was 38.13μg/ml,after treatment with polysaccharides from the Undaria Pinnatifida in different concentrations for 24 h.The cell apoptosis of BGC-823 cells were induced significantly and have a characteristic structure changes of apoptosis.The apoptotic ratio is 69.91% treated with 100 μg/ml of PUP,and the expression level of survivin mRNA were decreased significantly（P0.01）.Conclusion PUP can inhibit the proliferation,inducing apoptosis of BGC-823 cell.This effect was associated with the down-regulation expression of survivin gene.

关 键 词：胃癌 裙带菜多糖 细胞凋亡 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]