改良法兔VX2肝移植瘤模型建立及综合治疗的实验研究  

Experimental Study on the Retrofit Method of Building Transplanted Model of Rabbit Liver VX2 Tumor and the Effect of Combined Therapy

作　　者：李海青[1] 刘作勤[2] 

机构地区：[1]山东大学医学院,山东济南250012 [2]山东省医学影像学研究所

出　　处：《实用放射学杂志》2011年第2期270-275,共6页Journal of Practical Radiology

摘　　要：目的探讨DSA没备导引下经皮穿刺瘤组织块接种法制作兔VX2肝移植瘤模型的可行性及对比经导管动脉化疗栓塞（TACE）、经皮微波凝吲治疗（PMCT）及TACE＋PMCT3种方法治疗兔VX2肝移植瘤的效果。方法①新西兰大白兔50只，DSA设备导引下采用AngiotechSKATER穿刺针经皮穿刺接种瘤组织块于肝脏，14d后行CT平扫评价肿瘤接种成功率，测量肿瘤体积并随机处死10只实验兔进行病理分析。②取制作成功的肝移植瘤模型30只，随机分为TACE组（A组）、PMCT组（B组）和TACE＋PMCT组（C组），每组10只，治疗后第14天对比各组CT测量肿瘤体积、外周血CD3＋、CD4＋细胞分布、肿瘤组织坏死率。结果①DSA设备导引下经皮穿刺瘤组织块接种法制作兔VX2肝移植瘤模型成功率为100％。②治疗后14d，C组的肿瘤体积为（0．624±0．031）cm2，明显小于A组（O．799±0．057）cm3和B组（0．858±0．112）cm3，P〈0．05；C组外周血CD3＋细胞百分数为（66．30±1．36）％，明显高于A组（53．34±2．12）％和B组（60．68±1．06）％，P〈0．05；C组外周血CD4＋细胞百分数为（48．19±1．43）％，也明显高于A组（40．21±1．07）％和B组（44．03±0．08）％，P〈0．05；C组肿瘤组织坏死率明显高于A组和B组（P〈0．05）。结论①DSA设备导引下经皮穿刺瘤组织块接种法建立兔VX2肝移植瘤模型具有方法简单、成功率高、动物损伤小等特点。②TACE＋PMCT效果优于单纯经导管肝动脉化疗栓塞或微波凝固治疗。Objective To evaluate the feasibility in establishing the transplanted model of rabbit VX2 liver tumor using percutane- ous puncture tumor tissue pieces inoculation guided by the equipment of digital subtraction angiography（ DSA） and the treating effect for rabbit liver VX2 tumor by transcatheter arterial chemoembolization（TACE）, percutaneous microwave coagulation therapy（PM- CT）and TACE plus PMCT. Methods OVX2 tumor masses were implanted into the liver in 50 New Zealand white rabbits through Angio tech SKATER percutaneous puncture needle guided by the equipment of DSA, 14 days later,all rabbits underwent routine CT scan and the volumes of the VX2 tumor were measured them were killed to examine the tumors, histopathologically . （%）A total of 30 New Zealand white rabbits with liver transplanted VX2 tumor models was established successfully and were randomly divided into three groups（10 in each group） ：they treated by TACE（group A）,PMCT（group B）and TACE plus PMCT（group C） seperately. Fourteen days later,the volumes of the tumors, the distribution of CD3＋ and CD4＋ cell in peripheral blood and the necrostic rate of tumors between each group were compared. Results OThe successful rate of percutaneous puncture tumor tissue pieces inoculation to establish rabbit VX2 liver tumor model was 100 0/%. （%） Fourteen days after the treatment,the volume of the tumor of group C was （0. 624±0. 031）cm3 ,it had significant difference with that in group A （ 0. 799±0. 057 ） cm3 and group B （ 0. 858±0. 112）cm3 ,P〈 0.05. The percentage of CD34 cell in peripheral blood of group C was （ 66.30±1.36 ） %, it had significant difference with that in group A （ 53.34±2.12 ）% and group B （ 60.68±1.06 ）%,P〈0.05. The percentage of CD4＋ cell in peripheral blood of group C was（48. 19 ±1.43）%,it also had significant difference with that in group A（ 40.21±1.07 ） % and group B（ 44.03±0.08 ）% , P〈0.05. The necrostic rate of the tumors in group C had sign- ifica

关 键 词：肝 VX2肿瘤 介入放射学 

分 类 号：R735.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]