肺纤维化初期肺动脉高压大鼠肺动脉反应性的变化  

Alterations in pulmonary arterial reactivity during pulmonary arterial hypertension at the early-stage of pulmonary fibrosis in rats

作　　者：胡晓杰[1] 陈晓玲[1] 陈超[2] 艾洁[1] 李嘉[3] 韩晓静[1] 

机构地区：[1]河北医科大学基础医学研究所病理生理学研究室,石家庄050017 [2]河北医科大学药理研究室,石家庄050017 [3]河北医科大学第三医院关节科,石家庄050017

出　　处：《中国应用生理学杂志》2011年第1期110-114,139,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(30570789);河北省自然科学基金资助项目(C2004000582)

摘　　要：目的:观察肺纤维化初期肺动脉高压大鼠肺动脉血管反应性的变化。方法:66只雄性SD大鼠,随机分为博莱霉素(BLM)组和手术对照(Sham)组。BLM组为气管内一次性滴注BLM(5 mg/kg);Sham组为气管内滴注等容量的生理盐水(NS)。应用离体血管张力检测技术测定大鼠肺动脉血管反应性变化;用HE显示肺动脉壁病理形态学变化;Masson染色检测肺纤维化程度;右心漂浮导管技术测定大鼠平均肺动脉压。结果:①BLM组大鼠的肺动脉血管(保留内皮和去内皮)对苯肾上腺素(PE)的收缩反应均弱于Sham组(P均<0.05)。②BLM组大鼠肺动脉血管(保留内皮)对氯化乙酰胆碱(Ach)的舒张反应明显弱于Sham组(P<0.01)。③Sham组有内皮的肺动脉血管对L-NAME和PE联合作用的收缩反应明显强于PE单独作用(P<0.01),而BLM组有内皮肺动脉血管对L-NAME和PE联合作用的收缩反应与对PE单独作用比,其差异无统计学意义(P>0.05)。④BLM组肺动脉内皮细胞脱落。⑤BLM组大鼠肺组织呈现纤维增生初期的病理特征,且大鼠的平均肺动脉压明显高于Sham组(P<0.05)。结论:肺纤维化形成初期肺动脉高压大鼠肺动脉血管反应性出现异常。Objective： To explore the alterations in pulmonary arterial reactivity during pulmonary arterial hypertension at the early-stage of pulmonary fibrosis in rats.Methods： Sixty-six male Sprague-Dawley rats were randomly divided into 2 groups： bleomycin（BLM） group and sham group.The rats in BLM group were received single intratracheal instillation of BLM（5 mg/kg）,and the rats in sham group received equal volume of 0.9% normal saline（NS）.The alterations in pulmonary arterial reactivity were measured by vascular tension detected technique,the pathomorphological changes in the wall of pulmonary arteries were displayed with Hematoxylin-Eosin（HE） staining,the degree of fibrosis in lung was revealed with Masson staining,and the mean pulmonary arterial pressure was detected via a catheter in the pulmonary artery.Results： ①The contractile response to α-adrenoceptor agonist phenylephrine（PE）,of pulmonary arteries both with remaining endothelium and with removing endothelium,from BLM-treated rats,was reduced significantly,compared with sham rats（P both0.05）.②The relaxant response to the endothelially dependent vasodilator acetylcholine（Ach）,of pulmonary arteries with remaining endothelium,from BLM-treated rats,was also reduced,compared with sham rats（P0.01）.③In sham rats,the contractile response to（omega）-nitro-L-arginine methyl ester（L-NAME） plus PE,of pulmonary arteries with remaining endothelium,was enhanced,compared with that to PE alone（P0.01）,while in BLM group,the contractile responses to L-NAME plus PE,of pulmonary arteries with remaining endothelium,was not diffe-rent from that to PE alone（P0.05）.④In BLM group,vascular endothelial cells lost.⑤In BLM group,the initial stage of fibrogenesis was observed in lungs,and the mean pulmonary arterial pressure increased,compared with that in sham group（P0.05）.Conclusion： The abnormal responsibility of pulmonary arteries occurred during pulmonary arterial hypertension at the early-stage of pulmonary fibrosi

关 键 词：肺动脉 血管收缩 血管内皮依赖性舒张 博莱霉素 大鼠 

分 类 号：R363[医药卫生—病理学]