内吗啡肽在小鼠树突状细胞的诱导表达  

Inducible expression of endomorphins in murine dendritic cells

作　　者：李正红[1] 高琴[1] 叶红伟[1] 单立冬[2] 龚珊[2] 蒋星红[2] 

机构地区：[1]蚌埠医学院生理学教研室,安徽蚌埠233030 [2]苏州大学医学部神经生物学教研室,江苏苏州215123

出　　处：《中国药理学通报》2011年第1期77-81,共5页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学基金资助项目(No090413097);苏州大学中日校际合作研究资助项目(NoEE134005)

摘　　要：目的研究内吗啡肽-1和内吗啡肽-2在小鼠树突状细胞的表达。方法从7～8周龄的C57BL/6J小鼠骨髓提取骨髓前体细胞培养,经CD11c(树突状细胞的特异性标记)免疫磁珠分选,获得纯化的树突状细胞。流式细胞仪分析表明,CD11c阳性细胞纯度达95%以上。免疫荧光染色研究内吗啡肽-1和内吗啡肽-2在树突状细胞的表达;酶免疫测定(enzyme immunoassay,EIA)的方法,检测培养树突状细胞上清液中的内吗啡肽-1和内吗啡肽-2的含量。结果免疫荧光染色表明,活化的树突状细胞内可分泌内吗啡肽-1和内吗啡肽-2;酶免疫测定检测结果表明,不同的Toll样受体(toll like receptor,TLR)配体活化树突状细胞促使分泌不同浓度的内吗啡肽-1和内吗啡肽-2。结论活化的树突状细胞可诱导内吗啡肽的表达。Aim To study the expression of endomorphin（EM）-1 and EM-2 in dendritic cells（DCs）.Methods Bone marrow dendritic cells were extracted from 7-to 8-week-old C57BL/6J mice,purified with anti-CD11c（a specific marker for dendritic cells） antibody-coated magnetic beads and cultured in the presence of GM-CSF.FACS analysis indicated that the purity of DCs was more than 95%.Immunofluorescence staining was used to detect to expression of EMs in DCs.The enzyme immunoassay（EIA）was used to measure the contents of EMs.Results Immunofluorescence staining revealed that EM-1 and EM-2 were expressed and upregulated in DCs activated by LPS.Enzyme immunoassay showed that LPS and other Toll-like receptor（TLR）ligands promoted the secretion of EM-1 and EM-2 from activated DCs.Conclusions LPS can activate DCs and upregulate the expression of EM-1 and EM-2.TLR ligands can promote the secretion of EMs from DCs.

关 键 词：内吗啡肽-1 内吗啡肽-2 Mu阿片受体 树突状细胞 抗原递呈细胞 TOLL样受体 酶免疫测定 

分 类 号：R-332[医药卫生] R322.81