银杏内酯B对ApoE基因敲除小鼠动脉粥样硬化的影响  

Effect of ginkgolide B on atherosclerosis in ApoE^(-/-) mice

作　　者：刘熹昀[1] 赵革新[1] 鲍利[1] 陈北冬[1] 吴伟[1] 齐若梅[1] 

机构地区：[1]卫生部北京医院老年医学研究所卫生部老年医学重点实验室,北京100730

出　　处：《中国药理学通报》2011年第1期81-84,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题(No81070231;30572083)

摘　　要：目的探讨银杏内酯B(ginkgolide B,GB)对ApoE基因敲除(ApoE-/-)小鼠动脉粥样硬化的影响。方法 12只8周龄C57BL/6J小鼠为正常组,24只8周龄♂ApoE-/-小鼠为阳性模型组以及药物组;药物组每天给予GB 0.6 mg灌胃。8周后处死小鼠,用病理学方法观察小鼠动脉斑块、脂质沉积以及巨噬细胞表达。用ELISA方法测定血浆RAN-TES含量。结果电镜结果显示模型组动脉斑块较大,血管内膜损伤明显,而GB组小鼠动脉内表面损伤明显减轻,斑块较小。HE染色显示了同样的结果,模型组斑块较大,GB组斑块较小。血浆RANTES测定正常组为123.81 ng.L-1,模型组为359.16 ng.L-1,GB组为193.36 ng.L-1,各组之间差异存在统计学意义(P<0.01)。结论 GB能有效地减轻ApoE-/-小鼠的动脉粥样硬化损伤,并降低血浆RANTES含量,其药理学机制可能与抑制血小板功能相关。Aim To investigate the effect of ginkgolide B（GB） on the development of atherosclerosis in ApoE-/-defective mice.Methods Eight-week old C57BL/6J mice consisted of the normal group（n=12）.Eight-week old ApoE-/-mice were assigned randomly into two groups ： model group（fed with PBS 0.3 ml·d-1,n=12）and GB group（fed with GB 0.6 mg·d-1,n=12）.The mice were sacrificed after 8 weeks.The lesion of atherosclerosis was observed by electron microscope and pathological staining,respectively.Plasma level of RANTES was measured by ELISA kit.Results Electron microscope and hematoxylin-enosin staining showed that the area of atherosclerotic lesion was significantly less in GB-treated group than in model group.The result of aortic arch stained with oil red O showed lipid deposition was less in GB-treated mice than in the model group.Moreover,plasma RANTES was measured in the experiments.The level of RANTES was 123.81 ng·L-1 in the normal group,359.16 ng·L-1 in the model group and 193.36 ng·L-1 in GB-treated group,respectively.That indicated a significant difference of the efficacy of GB in each group（P0.01）.Conclusions Ginkgolide B can effectively relieve atherosclerosis lesion and decrease the level of RANTES in ApoE-/-mice.The mechanism might be associated with the inhibition of platelet function.

关 键 词：银杏内酯B APOE基因敲除小鼠 动脉粥样硬化 RANTES PAF受体拮抗剂 血小板释放 

分 类 号：R-332[医药卫生] R284.1