松果菊苷对脑缺血大鼠纹状体细胞外液中单胺类神经递质的影响  

Effects of echinacoside on striatal extracellular levels of monoamine neurotransmitter in cerebral ischemia rats

作　　者：魏丽丽[1] 陈虹[1] 姜勇[2] 屠鹏飞[2] 钟明[1] 杜娟[1] 刘斐[1] 刘春阳[1] 王磊[1] 

机构地区：[1]石河子大学药学院,教育部新疆特种植物药重点实验室,新疆石河子832002 [2]北京大学中医药现代研究中心,北京100083

出　　处：《中国药理学通报》2011年第2期174-177,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30860334,30560171)

摘　　要：目的研究松果菊苷(ECH)对局灶型脑缺血大鼠纹状体细胞外液中单胺类递质的影响,以探讨ECH对脑神经保护作用的可能机制。方法 SD大鼠随机分为对照组、模型组、ECH高、低剂量组和川芎嗪(CXQ)组。各组大鼠给予相应的药物或生理盐水腹腔注射,每天1次,连续7 d。给药第3天,脑纹状体埋置探针套管,末次给药1 h后,制作大鼠局灶性脑缺血模型(MCAO),造模后立刻进行微透析,将透析液注入高效液相-电化学检测器(HPLC-ECD)测定各组纹状体细胞外液中去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)、3,4-二羟苯乙酸(DOPAC)、高香草酸(HVA)、5-羟吲哚乙酸(HIAA)的含量。结果与对照组相比,模型组NE、DA、5-HT水平升高,其酸性代谢产物DOPAC,HVA,HIAA也随之升高。与模型组比较,ECH高、低剂量组(30、15 mg.kg-1.d-1)和CXQ组的6种物质的含量均有所降低。结论 ECH的神经保护作用可能与对抗脑缺血后单胺类神经递质的升高有关。Aim To examine the effects of ECH on striatal extracellular levels of NE,DA,DOPAC,HIAA,HVA,5-HT in cerebral ischemia rats and its possible mechanisms of neuro-protective effect.Methods Rats were divided into control,model,ECH high and low dose and CXQ groups randomly.Every rat was administered drugs or vehicle through introperitoneal injection,one time a day for seven consecutive days.At day 3 focal ischemia was generated by permanent middle cerebral artery occlusion（MCAO）.Then the striatal extracellular fluids were gained by brain microdialysis.The methods of high performance liquid chromatography with electrochemical detection were used to measure the striatal extracellular levels of NE,DA,DOPAC,HIAA,HVA,5-HT.Results The results showed that the level of NE,DA,5-HT increased rapidly,and sodid its metabolites DOPAC,HIAA,HVA accordingly.Compared with model,administration of ECH and CXQ successfully prevented the extracellular levels of NE,DA,DOPAC,HIAA,HVA,5-HT from elevation induced.Conclusion ECH can reduce monoamine neurotransmitters and their metabolites content of striatal extracellularlevels,which may be one of the mechanisms of anti-cerebral ischemia.

关 键 词：松果菊苷 脑缺血 纹状体 HPLC-ECD 单胺类神经递质及代谢产物 

分 类 号：R-332[医药卫生] R284.1