180份玉米自交系亲缘关系的分子评价  

Molecular Evaluation of 180 Maize Inbred Lines by SSR Markers

作　　者：乔治军[1] 刘龙龙[1] 南晓洁[1] 赵秀娟[1] 王海岗[1] 

机构地区：[1]山西省农业科学院农作物品种资源研究所,太原030031

出　　处：《植物遗传资源学报》2011年第2期211-215,222,共6页Journal of Plant Genetic Resources

基　　金：作物种质资源保护项目(NB08-2130135-(25-31)-11)

摘　　要：利用覆盖玉米全基因组的22对SSR引物,对180份玉米自交系的亲缘关系进行分子评价。结果显示:22对SSR引物共检测到129个等位基因,每一位点平均等位基因数5.9,变幅2～13;平均基因多样性指数和平均多态性信息含量分别为0.583和0.528。基于模型的群体结构分析将所有材料分为5个类群,与国内自交系划分的杂种优势群体相一致。AMOVA分析表明,5个类群的遗传变异主要存在于群体内,群体间的遗传变异仅占总变异的3.38%,且达到极显著水平。不同群体间的遗传分化在0.021～0.079之间。22 SSRs covered the entire maize genome were selected to fingerprint a set of 180 maize inbred lines preserved.Totally,129 alleles were detected by these 22 SSRs,averaged 5.9 per locus,ranging from 2 to 13.The average gene diversity was 0.583,and the overall PIC across all these SSRs was 0.528.Model-based clustering analysis indicated that all these 180 inbreds could be seperated into 5 distinct groups,agreed well with the heterotic groups of Chinese maize inbreds.Results from the F-statistics and the analysis of molecular variance（AMOVA） showed that the genetic differentiation coefficient among model-based groups varied from 0.021 to 0.079,and the total genetic variation among these groups only accounted for 3.38%,the rest（96.62%） was observed existing within these groups.

关 键 词：玉米自交系 SSR标记 遗传多样性 遗传结构 

分 类 号：S513[农业科学—作物学]