三七栽培居群遗传多样性的EST-SSR分析  

Genetic Diversity Analysis of Populations of Panax notoginseng Using EST-SSR Markers

作　　者：张金渝[1,2] 杨维泽[1] 崔秀明 金航[1] 虞泓[2] 陈中坚 沈涛[1,2] 杨涛[1] 

机构地区：[1]云南省农业科学院药用植物研究所,昆明650223 [2]云南大学生命科学学院生态遗传实验室,昆明650091 [3]文山三七科学院,云南文山663000

出　　处：《植物遗传资源学报》2011年第2期249-254,共6页Journal of Plant Genetic Resources

基　　金：国家“十一五”科技支撑项目(2006BAI06A12-13);国家“十二五”科技支撑项目(2011BAI13B01);云南省自然科学基金项目(2007C136M)

摘　　要：利用EST-SSR标记分析比较6个文山三七居群的遗传多样性和遗传结构,并以2个近缘种为对照进行聚类分析。17对人参属EST-SSR引物在8个居群中共检测到205个多态位点。在居群水平上,6个三七栽培居群的平均多态性信息量为0.729,Ne i′s基因多样性为0.1568,Shannon多样性指数为0.2466,居群间的遗传分化系数为0.2350。研究显示三七具有丰富的遗传多样性,但彼此间具较高的基因交流,居群间遗传分化水平低,遗传差异主要存在于居群内;另外,遗传相似度和聚类分析显示,三七及其近缘种被划分为3个大类群,6个三七栽培居群被分为3个小类群,三七与珠子参有较近的遗传关系,而与屏边三七的遗传距离较远。The genetic diversity and genetic structure of 6 populations of Panax notoginseng and its two related species were analyzed by using 17 pairs of EST-SSR molecular markers in this research.As a result,a total of 205 EST-SSR polymorphic loci were detected in 8 populations.The polymorphism information content of six cultivated populations of P.notoginseng was 0.729,Gene diversity within population was 0.1568,Shannon＇s information index was 0.2466,and the coefficient of gene differentiation was 0.2350 among populations of P.notoginseng.The coefficient of gene differentiation was 0.4597 between P.notoginseng and its related species.The results revealed abundant genetic diversity but low level of genetic differentiation in P.notoginseng and confirmed that the genetic difference amang the species existed mainly within population.In addition,genetic similarity and cluster analysis showed that 8 populations of P.notoginseng and its related species were classified into three groups,6 populations of P.notoginseng were classified into three subgroups.Genetic distance was closer between P.notoginseng and P.japonicus var.major than that between P.notoginseng and P.stipuleanatus.

关 键 词：三七 EST-SSR标记 遗传多样性 遗传结构 

分 类 号：S567.236[农业科学—中草药栽培]