石歧杂鸡催乳素基因外显子4变异与产蛋就巢性状的关联分析  

SNP Detection of Exon 4 of Prolactin Gene and its Associations with Egg-laying and Broodiness Traits in Shiqiza Chicken

作　　者：逯岩[1,2] 李福伟[1,2] 曹顶国[1,2] 雷秋霞[1,2] 韩海霞[1,2] 周艳[1,2] 李桂明[1,2] 黄保华[1,2] 

机构地区：[1]山东省农业科学院家禽研究所,山东济南250023 [2]山东省家禽育种工程技术研究中心,山东济南250023

出　　处：《中国畜牧兽医》2011年第3期146-149,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家行业科技计划(3-46;3-26);山东省良种工程项目(2009LZ014);现代农业产生技术体系建设专项资金资助(nycytx-41-z09);山东省农业科学院创新基金会(2007-ycx013)

摘　　要：利用PCR-SSCP技术和测序技术对催乳素(prolactin,PRL)基因外显子4进行SNP检测,并分析了该基因与石歧杂鸡产蛋、就巢性状的相关性。结果表明,PRL基因外显子4存在1个多态性位点,该位点在6017位点发生G→A的碱基突变,产生了AA、AB和BB 3种基因型,AA基因型为优势基因型,等位基因A为优势等位基因,该突变未导致氨基酸的变异;该位点的变异与开产日龄、首次就巢日龄呈现一定的显著差异趋势,BB基因型开产日龄、首次就巢日龄都晚于AA和AB基因型。Exon 4 of prolactin gene were detected by PCR-SSCP and the DNA fragments were sequenced.The correlation analysis between the polymorphism of the exon 4 and egg-laying and broodiness traits were carried out respectively.The results indicated that there were one polymorphic sites in exon 4.A G→A mutation at the position of the 6017 bp was found,which resulted in 3 genotypes（AA,AB and BB）,but the mutation did not lead to changes in amino acid.The frequencies of genotype AA and allele A were the highest.The least square analysis showed that the first broodiness day and age at 1st egg of BB type chickens was later than AA and AB types chickens,The locus of variation showed a definite trend in the age at 1st egg and the first broodiness day.

关 键 词：PRL基因 PCR-SSCP 石歧杂鸡 就巢性状 

分 类 号：S831[农业科学—畜牧学]