美加明对人肺腺癌A549细胞增殖和迁移的影响  

Effects of mecamylamine on cell proliferation and migration of human lung adenocarcinoma A549 cells

作　　者：马晓丽[1,2,3] 孙海基[3] 赵云[1,2] 汪运山[1,2] 安利国[3] 

机构地区：[1]山东大学附属济南市中心医院医学实验诊断中心,济南250013 [2]山东省肿瘤防治靶向分子研究重点实验室,济南250013 [3]山东师范大学生命科学学院生理学教研室,济南250014

出　　处：《肿瘤》2011年第1期39-43,共5页Tumor

基　　金：中国博士后科学基金资助项目(编号:200902575);济南留学人员创业计划资助项目(编号:20080407)

摘　　要：目的:探讨烟碱型胆碱能受体(nicotinic acetylcholine receptor,nAChR)拮抗剂美加明对人肺腺癌A549细胞增殖和迁移的影响。方法:应用磺酰罗丹明B(sulforhodamine B,SRB)法和FCM法检测美加明对人肺腺癌A549细胞增殖、细胞周期和细胞凋亡的影响;划痕实验检测美加明对细胞迁移能力的影响;激光共聚焦显微镜下观察美加明对A549细胞E-钙黏蛋白(E-cadherin,E-cad)表达的影响。结果:美加明可抑制A549细胞的增殖,与对照组比较差异有统计学意义(P<0.05),并且其抑制作用随着药物浓度的增加而增强。FCM检测结果显示,美加明作用后,G0/G1期细胞比率增多,S期和G2/M期细胞比率下降,而细胞凋亡未发生明显变化。划痕实验结果显示,美加明可降低A549细胞的迁移能力。激光共聚焦显微镜下观察发现,美加明可上调A549细胞E-cad的表达。结论:美加明可能通过阻滞细胞周期于G0/G1期而抑制A549细胞增殖,并通过上调E-cad的表达而降低细胞迁移。Objective： To study the effects of nicotinic acetylcholine receptor （nAChR} antagonist mecamylamine on cell proliferation and migration of human lung cancer A549 cells. Methods： Sulforhodamine B （SRB） and flow cytometry （FCM） were used to detect the proliferation, cell cycle and apoptosis of A549 cells after treatment with mecamylamine. Wound healing test was used to measure the migration ability of A549 cells. The expression of E-cadherin （E-cad） was observed under a laser scanning confocal microscope. Results： Compared with negative control cells, the proliferation of A549 cells was inhibited by mecamylamine treatment in a dose-dependent manner （P〈0.05） . The percentage of Go/G1 phase cells increased while the S and G2/M phase cells decreased after treating with mecamylamine. There was no change observed in cell apoptosis. The migration capacity of mecamylamine-treated cells was decreased compared with that of the negative control cells. The expression of E-cad was up-regulated in A549 cells treated with mecamylamine. Conclusion： Mecamylamine inhibits the cell proliferation and decreases the migration ability of A549 cells. These effects are related with blocking A549 cells into Go/G1 phase and up-regulating E-cad expression.

关 键 词：肺肿瘤 美加明 细胞 培养的 细胞增殖 细胞运动 烟碱型胆碱能受体 细胞 A549 

分 类 号：R734.2[医药卫生—肿瘤]