核因子-κB/p65表达下调对舌鳞状细胞癌裸鼠移植瘤中bcl-2和bax表达的影响  

Effects of NF-B/p65 siRNA on expressions of bcl-2 and bax in xenografted human tongue squamous cell carcinoma

作　　者：路学文[1] 解邦杰[1] 李新明[2] 岳增文[1] 蔡留意[1] 

机构地区：[1]武警河南总队医院口腔科,郑州450052 [2]郑州大学第一附属医院口腔科,郑州450052

出　　处：《肿瘤》2011年第1期44-48,共5页Tumor

摘　　要：目的:本实验旨在探讨下调核因子-κB(nuclear factor-kappa B,NF-κB)/p65亚单位的表达对舌鳞状细胞癌裸鼠移植瘤生长及细胞凋亡相关基因bcl-2和bax表达的影响。方法:应用舌鳞状细胞癌Tca8113细胞建立舌鳞状细胞癌裸鼠移植瘤模型。将针对p65的小干扰RNA(small interfering RNA,siRNA)接种荷瘤裸鼠,观察下调NF-κB/p65表达后荷瘤裸鼠的生长情况;采用TUNEL法检测肿瘤组织中的细胞凋亡情况;进一步应用RT-PCR和Western印迹法检测肿瘤组织中p65、bcl-2和bax的mRNA及蛋白表达变化。结果:经p65siRNA治疗后,肿瘤的生长明显受到抑制(P<0.05);TUNEL结果表明,p65siRNA组肿瘤细胞明显发生凋亡;此外,p65siRNA组裸鼠肿瘤组织p65和bcl-2的mRNA及蛋白表达明显下调,而bax的mRNA及蛋白表达显著上调,差异均有统计学意义(均P<0.05)。结论:NF-κB/p65亚基可能在舌鳞状细胞癌的细胞凋亡中发挥重要作用。Objective： To investigate the effects of expression downregulation of nuclear factor-kappa B （NF-KB）/p65 on the growth of tumor and expressions of apoptosis-related genes bcl-2 and bax in xenografted tongue squamous cell carcinoma. Methods： Tongue squamous cell carcinoma Tca8113 cells were subcutaneously inoculated into BALB/c nude mice to establish the xenografted model. The growth of xenografted tumor was observed after treatment with p65 small interfering RNA （siRNA）. The apoptosis of xenografted tumor cells in nude mice was detected by TUNEL assay. Expressions of p65, bcl-2 and bax mRNAs as well as their proteins were detected by RT-PCR and Western blotting. Results： The growth of xenografted tumors in p65 siRNA group was obviously inhibited compared with those of the control group and control-siRNA group （P〈0.05）. The result of TUNEL assay demonstrated that p65 siRNA evidently evoked cell apoptosis. The expressions of p65 and bcl-2 mRNAs as well as their proteins in p65 siRNA group were significantly downregulated, but the expression of bax was markedly upregulated. There were significant differences among three groups （P〈0.05）. Conclusion： NF-KB/p65 subunit may play an essential role in cell apoptosis of tongue squamous cell carcinoma.

关 键 词：舌肿瘤 肿瘤 鳞状细胞 NF-KB 癌基因蛋白质p65 细胞凋亡 BCL-2相关X蛋白质 小鼠 近交BALB C 

分 类 号：R739.8[医药卫生—肿瘤]