人卵巢上皮性癌组织中Pim-1与C-myc的表达及其临床意义  

Expressions of Pim-1 and C-myc in human ovarian epithelial cancer and their clinical significance

作　　者：刘芳梅[1] 苑中甫[1] 

机构地区：[1]郑州大学第一附属医院妇产科,郑州450052

出　　处：《肿瘤》2011年第1期69-73,共5页Tumor

摘　　要：目的:探讨人卵巢上皮性癌组织中Pim-1和C-myc的表达及其临床意义。方法:采用RT-PCR半定量方法和免疫组织化学法检测40例卵巢上皮性癌组织、20例卵巢上皮性良性肿瘤组织和10例正常卵巢组织中Pim-1和C-myc mRNA及蛋白的表达情况。结果:卵巢上皮性癌组织中Pim-1和C-myc mRNA与蛋白的表达水平明显高于卵巢上皮性良性肿瘤组织及正常卵巢组织(P<0.05);Pim-1蛋白在卵巢上皮性癌组织中的表达与其组织学类型无关(P>0.05),与国际妇产科联盟(Federation International of Gynecology and Obstetrics,FIGO)分期和淋巴结转移有关(P<0.05);C-myc蛋白在卵巢上皮性癌组织中的表达与其组织学类型、FIGO分期和淋巴结转移均无关(P>0.05)。结论:原癌基因Pim-1和C-myc的mRNA及蛋白在卵巢上皮性癌组织中过度表达,可能与卵巢上皮性癌的发生、发展有关。Objective： To investigate the expressions of Pim-1 and C-myc in human ovarian epithelial cancer, and to elucidate their clinical significance. Methods： The mRNAand protein expressions of Pim-1 and C-myc were evaluated by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemistry method in 40 ovarian epithelial cancer tissues, 20 benign ovarian epithelial tumor tissues and 10 normal ovarian tissues. Results： The expression levels of Pim-1 and C-myc mRNAs as well as their proteins in ovarian epithelial cancer tissues were higher than those in benign ovarian epithelial tumor tissues and normal ovarian tissues （P〈0.05） .The protein expression of Pim-1 did not correlate with histological classification （P〉0.05） , but correlated with the Federation International of Gynecology and Obstetrics （FIGO） staging and lymph node metastasis of ovarian cancer （P〈0.05） . The protein expression of C-myc did not correlate with histological classification, FIGO staging and lymph node metastasis of ovarian cancer （P〉0.05） .Condusion： The over-expressions of both mRNAs and proteins of pro- oncogene Pim-1 and C-myc may be involved in the initiation and progression of ovarian epithelial cancer.

关 键 词：卵巢肿瘤 基因表达 反转录聚合酶链反应 免疫组织化学 基因 Pim-1 基因 C-MYC 

分 类 号：R737.31[医药卫生—肿瘤]