磷酸化转录激活因子5在胰腺癌细胞的表达及生长激素的干预作用  

Expression of pSTAT5 in pancreatic carcinoma cells and effect of growth hormone on pSTAT5

作　　者：石毅[1] 孙跃明[1] 白剑锋[1] 陆文熊[1] 傅赞[1] 奚春华[1] 赵翰林[1] 苗毅[1] 

机构地区：[1]南京医科大学第一附属医院普外科,江苏南京210029

出　　处：《中华胰腺病杂志》2011年第1期34-36,共3页Chinese Journal of Pancreatology

摘　　要：目的检测磷酸化转录激活因子5（pSTAT5）在7株胰腺癌细胞株中的表达，观察生长激素（GH）处理SW1990细胞及其移植瘤后pSTAT5表达的变化，探讨GH的分子作用机制。方法体外培养人胰腺癌细胞株SW1990、Cap-1、Colo、Mia、Aspc、P3、PANC1，Western blotting检测各株细胞的pSTAT5表达；收集指数生长期的SW1990细胞接种于BALB／C裸鼠，成瘤后随机分为GH组（瘤内注入GH4mg·kg^-1·d^-1，连续2周）和对照组（NS组），最后一次注射GH后1、2、24h分批处死裸鼠，Western blotting检测SW1990细胞和移植瘤pSTAT5蛋白表达的变化。结果所有胰腺癌细胞（SW1990、Cap-1、Colo、Mia、Aspc、P3、PANC1）均有pSTAT5表达。GH（50ng／ml）刺激后5min，SW1990细胞pSTAT5的表达量为0．57±0．05，较刺激前显著增高，10min达到0．64±0．04，15min很快下降至0．39±0．03，但直至1h仍高于对照组（0．33±0．02对0．25±0．06），2h后表达量为0．26±0．03，回落到基础水平。移植瘤pSTAT5表达无明显变化。结论GH可迅速上调SW1990细胞的pSTAT5表达，但维持时间较短，而对胰腺癌移植瘤pSTAT5的表达无显著影响。Objective To investigate the expression of pSTAT5 in 7 pancreatic carcinoma cell lines, and the change of expression of pSTAT5 in pancreatic carcinoma cells SW1990 after growth hormone （GH） treatment, and explore its molecular mechanism. Methods Human pancreatic carcinoma cell lines （SW1990, Cap-1, Colo, Mia, AsPc, P3, PANC1） were cultured in vitro, and Western blotting was used to detect the expression of pSTAT5 in these cell lines. SW1990 in exponential growth phase was collected and nude Balb/e mice were inoculated with SW1990 cells. When tumors became palpable after inoculation, mice were randomized to receive GH （ intratumorally 4 mg· kg^- 1· d^ - 1 once daily for 2 weeks） and control group （normal saline group）. 1 h, 2 h and 24 h after the last dose of GH treatment, the mice were sacrificed. Western blotting was used to detect the expression of pSTAT5 in SW1990 and inoculation tumor cells after GH injection. Results Positive expression of pSTAT5 was observed in all human pancreatic carcinoma cell lines （SW1990, Cap-1, Colo, Mia, Aspc, P3, PANC1）. 5 minutes after GH （50 ng/ml） stimulation, the expression of pSTAT5 in SW1990 was 0.57 ± 0.05, which was significantly increased ; and it reached 0. 64 ± 0.04 at 10 minutes, then decreased to 0.39 ±0.03 at 15 minutes, however, it remained higher than that in the control group at 1 h （0.33 ± 0.02 vs 0.25 ± 0.06）, and its expression at 2 h was 0.26 ± 0.03 and returned to the normal level. The expression of pSTAT5 in xenograft was not significantly changed. Conclusions GH could rapidly up-regulate the expression of pSTAT5 in SW1990 but the effect lasted for a relatively short period. GH had no significant effect on the expression of pSTAT5 in xenograft.

关 键 词：胰腺肿瘤 STAT5转录因子 生长激素 信号转导 

分 类 号：R735.9[医药卫生—肿瘤]