超声引导脑室内神经干细胞移植治疗小儿脑瘫  

Transplantation of neural stem cells through the lateral ventricle under the guidance of ultrasound in children with cerebral palsy

作　　者：贺声[1] 栾佐[1] 屈素清[1] 张云山[1] 邱旋[1] 贾文凯[1] 沈燕华[1] 辛大庆[1] 李馨[1] 徐涛[1] 王苗苗[1] 

机构地区：[1]中国人民解放军海军总医院超声科,北京100048

出　　处：《中国介入影像与治疗学》2011年第2期94-97,共4页Chinese Journal of Interventional Imaging and Therapy

摘　　要：目的探讨超声引导下经皮侧脑室穿刺脑室内神经干细胞（NSCs）移植治疗脑瘫的方法及近期疗效。方法 45例脑瘫患儿接受超声引导下经皮穿刺脑室内NSCs移植术。NSCs来自自然流产的孕12周人胚胎前脑组织。采用常规彩色多普勒超声诊断仪,普通扇扩探头,频率2.0~4.0 MHz,带有穿刺架和引导软件。结果 45例患儿共接受56次超声引导下侧脑室穿刺,1次穿刺成功率为100%,脑室内注入NSCs 0.3~1.2 ml,平均（0.8±0.5）ml,细胞计数3.8×106~7.2×107个,平均（12.0±6.8）×106个;3例患儿有轻度脑室血管损伤,14例术后出现发热,经对症处理体温恢复正常。术后21例（21/45,46.67%）近期临床表现有不同程度好转,无术后病情加重病例。结论超声引导下脑室内NSCs移植术安全、准确、穿刺成功率高,有助于改善脑瘫患儿的临床症状。Objective To assess the course of percutaneous puncture of the lateral ventricle guided with ultrasonography,and to observe the short-term effectiveness of transplantation of human fetal neural stem cells（NSCs） into the cerebral ventricle in treatment of cerebral palsy（CP） of children.Methods Forty-five children of CP with different causes underwent transplantation of NSCs through the lateral ventricle guided with ultrasonography.NSCs were obtained from the forebrain of 12-week old fetuses because of natural abortion.Color Doppler ultrasound with sector detector,puncture frame and guidance software were used.Results Fifty-six taps were carried out and 0.3—1.2 ml（mean [0.8±0.5]ml） suspensions containing 3.8×106—7.2×107（mean [12.0±6.8]×106） NSCs were transplanted into the cerebral ventricles.The success rate of once puncture was 100%,during which mild injury of lateral ventricle blood vessel of the children occurred in 3 cases.Though 14 cases had fever,short-term clinical manifestations were improved in varying degrees in 21 cases（21/45,46.67%） after the transplantation.No patient＇s condition worsened.Conclusion Transplantation of NSCs into the lateral ventricle guided with ultrasonography is safe and accurate,and helps to improve the clinical symptoms for children with CP.

关 键 词：脑性瘫痪 儿童 侧脑室 穿刺术 干细胞移植 超声检查 

分 类 号：R742.3[医药卫生—神经病学与精神病学]