大鼠大脑中动脉梗塞模型的同步辐射活体血管影像学研究  

Application of Synchrotron Radiation Microangiography on Rat Middle Cerebral Artery Occlusion Models

作　　者：袁法磊[1] 管永靖[1,2] 肖体乔 谢红兰 杨国源[1,2] 王永亭[1] 

机构地区：[1]上海交通大学Med—X研究院,上海200030 [2]上海交通大学医学院附属瑞金医院,上海200025 [3]中科院上海应用物理研究所,上海201204

出　　处：《实验动物与比较医学》2011年第1期11-15,共5页Laboratory Animal and Comparative Medicine

基　　金：国家重点基础研究发展计划（973计划）（2010CB834306）;上海市科委实验动物研究专项（课题编号09140902400）;上海交通大学SMC-晨星青年学者奖计划

摘　　要：目的通过同步辐射微血管造影研究大鼠大脑中动脉梗塞脑缺血模型。方法20只300～350gSD大鼠平均分为两组：4．0线组和4—0线包裹硅橡胶组。施行大脑中动脉缺血手术后，立即进行同步辐射血管成像。结果单纯用4—0线组大鼠大脑中动脉没有发生完全阻塞，大脑前动脉和颈内动脉的尚有部分血供；4—0线包裹硅橡胶组，大鼠大脑中动脉均成功阻塞。10％大鼠具有两侧的孔型大脑中动脉（2／20只）。栓线进入深度17mm能够有效阻塞这种异常的大鼠大脑中动脉。结论包裹硅橡胶的4—0线栓能够成功造成300—350g大鼠大脑中动脉缺血，单纯4—0栓线无法完全造成这一体重范围内的大鼠缺血。由于同步辐射可以进行大鼠的脑血管造影，因此可适合研究和大鼠线栓法缺血模型以及评价大鼠脑部血管变异。Objective To apply synchrotron radiation microangiography on the study of rat middle cerebral artery occlusion （MCAO） models. Method Twenty Sprague-Dawley rats weighing 300-350 g were divided into two groups： 4-0 suture group and 4-0 silicone-coated suture group. Immediately after MCAO surgery, synchrotron radiation angiography was conducted. Result The plain 4-0 nylon suture failed to completely occlude the middle cerebral artery （MCA） of the rat. There was still blood supply from anterior cerebral artery （ACA） and interior carotid artery （ICA）. The silicone-coated 4-0 nylon suture successfully induced rat MCAO with 10% rats had fenestrated MCAs （2/20）.The variants in these rats were occluded when the suture depth was 17 mm. Conclusion The silicone-coated 4-0 nylone suture successfully induced MCAO in rats weighing from 300 to 350 g, and the plain 4-0 suture was not able to induce complete ischemia in these rats. Synchrotron radiation microagiography is useful for studying rat MCAO models and evaluating rat cerebral abnormalities.

关 键 词：血管造影 缺血模型 大鼠 同步辐射 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]