采用动态热回流法制备莲花菌多糖  

Preparation of polysaccharides from Grifola frondosa using dynamic hot reflux extraction

作　　者：赵永亮[1] 侯启昌[2] 李磊[1] 郭家瑞[1] 崔羽佳[1] 李鹏[1] 王卫国[1] 

机构地区：[1]河南工业大学生物工程学院,河南郑州450001 [2]河南广播电视大学,河南郑州450003

出　　处：《中南大学学报（自然科学版）》2011年第1期38-45,共8页Journal of Central South University:Science and Technology

基　　金：河南工业大学引进人才专项(2007BS023);河南省科技厅攻关计划项目(0124060026;0124060011)

摘　　要：以莲花菌子实体为材料,采用动态热回流法制备莲花菌多糖。通过单因素试验和正交试验研究原料颗粒粒径、提取温度、溶液pH、液料比、溶剂回流率、提取时间对莲花菌多糖提取效果的影响,并将动态热回流法与传统热水浸提法进行比较。研究结果表明:动态热回流法制备莲花菌多糖的最佳工艺条件是:原料颗粒粉碎度为3 500μm,提取温度为95℃,溶液pH为6.5,液料比为6.5-1.0,溶剂回流率为15%,提取时间为90 min,在此条件下,粗多糖得率可以达到13.98%,比传统热水浸提法提高16.1%,提取时间比传统法减少了3/4,溶剂用量比传统法减少了7/8。该方法可用于莲花菌多糖的规模化制备生产,亦可为其他真菌多糖及相关产品的大规模工业生产提供借鉴。A novel method of dynamic hot reflux extraction for the preparation of polysaccharides from the fruitbody of Grifolafrondosa was researched. The factors that affect the extraction of maitake polysaccharides including size of raw material particles, extraction temperature, pH, mass ratios of water to material, reflux rate of solvent and extraction time were studied by single factor test and orthogonal test. The extraction effect of dynamic hot reflux method was compared with that of traditional hot water distilling extraction. The results show that the optimal conditions of extraction with dynamic hot reflux technology are as follows： The size of raw material particles is 3 500 gm in diameter, extraction temperature is 95 ℃, mass ratio of water to material is 6.5：1.0, pH=6.5, reflux rate of solvent is 15%, extraction time is 90 rain. In the optimal conditions, the maitake polysaccharides extraction rate of dynamic hot reflux method reaches 13.98% and rises by 16.1% compared with traditional hot water distilling extraction. And this novel method can shorten 3/4 time of extraction and save 7/8 of the extraction solvent. The method not only makes large-scale industrial production of maitake polysaccharides, but also can be a reference for large-scale industrial production of other funsal polvsaccharides.

关 键 词：莲花菌(灰树花) 多糖 动态热回流法 

分 类 号：Q81[生物学—生物工程] Q93