人类表皮生长因子受体-2和拓扑异构酶Ⅱ在卵巢上皮癌中的表达及其临床意义  

Expression of HER-2 and TOPO- Ⅱα in ovarian epithelial cancer and its clinical significance

作　　者：尚素霜[1] 张静[1] 程佳[1] 任月环[1] 张利玲[1] 潘丽霞[1] 高翔[1] 于华[1] 

机构地区：[1]河北省邯郸市第一医院妇科,056002

出　　处：《中国综合临床》2011年第3期302-305,共4页Clinical Medicine of China

摘　　要：目的探讨原癌基因人类表皮生长因子受体也（HER-2）和拓扑异构酶Ⅱ（TOPO-Ⅱα）在卵巢上皮癌组织中的表达及其相关性。方法采用免疫组化SP方法检测经病理确诊的58例卵巢上皮癌、20例卵巢良性上皮肿瘤及10例正常卵巢组织标本中HER-2及TOPO-Ⅱα的表达情况，并结合临床病理特征进行分析。结果HER-2在卵巢正常组织、卵巢良性上皮性肿瘤组织中的阳性表达率分别为10．0％（1／10）、15．0％（3／20），显著低于卵巢上皮癌组织[46．6％（27／58），）（2值分别为4．705、6．225，P均〈0．05]；卵巢上皮癌组织中TOPO-Ⅱα的阳性表达率显著高于卵巢正常组织和卵巢良性上皮性肿瘤组织[53．4％（31／58）、10．0％（1／10）、15．0％（3／20），X^2值分别为6．463、8．941，P均〈0．05]；而良性肿瘤组织和正常组织间比较差异均无统计学意义（P均〉0．05）。HER-2和TOPO—Ⅱα的表达与卵巢上皮癌临床分期（X^2值分别为5．295、6．665）、细胞组织学分化（X^2值分别为5．376、8．981）有显著相关性（P均〈0．05），而与年龄、组织学类型无相关性（P均〉0．05）；卵巢上皮癌中HER-2和TOPO-Ⅱα蛋白的表达呈正相关（r8=0．324，P〈0．05）。结论HER-2在卵巢上皮癌的发生发展中起重要作用，对临床诊断及预后评估具有一定的指导意义。Objective To observe the expression of HER-2 and TOPO-Ⅱα in ovarian epithelial cancer,analyze the correlation between their expression and provida theoretical basis for clinical diagnosis, prognosis and treatment. Methods Expression levels of HER-2 and TOPO- Ⅱα in 10 normal ovarian tissues,20 benign tumors and 58 cases of ovarian epithelial cancers were detected by immunohistochemical method, and their correlations with pathological features were analyzed. Results The positive expression rate of HER-2 in normal ovarian and benign tumor tissues were significantly lower than ovarian epithelial cancers respectively （ 10. 0%, 15.0% VS 46. 6% ; P 〈0. 05 ）. The positive expression rate of TOPO- Ⅱα in ovarian epithelial cancers was significantly higher than normal and benign epithelial ovarian tumor tissue （53.4% vs 10. 0% ,and 15. 0%, Ps 〈0. 05）：but we did not find significant difference in the comparison between normal and benign epitlielial ovarian tumor tissue （ Ps 〉 0. 05 ）. The expression of HER-2 and TOPO- Ⅱα were significantly correlated with clinical stages, histological differentiation of tumor cells （ Ps 〈 0. 05 ）, but there were no correlations between the age or histological type. In ovarian cancer tissues, a positive correlation between the expression of HER-2 and TOPO- Ⅱα was observed （ r = 0. 324, P 〈 0. 05 ） . Conclusion The overexpression of HER-2 and TOPO- Ⅱα play an important role in ovarian carcinogenesis and development. The expression of HER-2 is positively correlated with TOPO- Ⅱα in ovarian epithelial cancers. Coexpression of the two moleculars may be involved in the develonment and oroa-ression of ovarian enithelial cancer,which shnuld be furlher studied.

关 键 词：卵巢上皮癌 人类表皮生长因子受体-2 拓扑异构酶Ⅱ 免疫组织化学 

分 类 号：R737.9[医药卫生—肿瘤]