低浓度一氧化碳对小鼠神经行为功能的影响  

Effects of low Concentration Carbon Monoxide on the Neurobehavior Function in Mice.

作　　者：梁宏[1] 张恒太[1] 于芳[1] 余秉良[1] 

机构地区：[1]航天医学工程研究所,北京100094

出　　处：《航天医学与医学工程》1999年第5期318-322,共5页Space Medicine & Medical Engineering

摘　　要：目的研究低浓度一氧化碳（ Ｃ Ｏ） 的神经行为毒理学效应及限时最大容许浓度（ Ｍ Ａ Ｃ） 。方法４０ 只昆明小鼠随机等分为４ 组（ ｎ ＝ １０） ： Ａ 组（ 对照组，吸入空气） ， Ｂ 组（ 吸入３０ ｍｇ／ ｍ ３ Ｃ Ｏ） ， Ｃ 组（ 吸入３００ ｍ ｇ／ ｍ ３ Ｃ Ｏ） ， Ｄ 组（ 吸入６００ ｍｇ／ ｍ ３ Ｃ Ｏ） 。各组分置于０ ．５ ｍ ３ 容积的静式染毒柜中，持续染毒７２ ｈ 。在染毒前、中、后，测试小鼠在水迷宫的反应时和错误率及在开阔场的走格子数和排便次数。结果 染毒期间 Ｄ 组的竖立次数明显少于其他三组。脱离染毒环境后组间比较，反应时和错误率在第４ ～６ 天（６ ｄ） 出现与染毒前相反的变化，第７ ～９ 天（９ ｄ） 的错误率数值大小为： Ｄ 组＞ Ｃ 组＞ Ａ 组＞ Ｂ 组，反应时数值变化与错误率变化趋同；走格子数，第１ ～３ 天（３ ｄ） Ｃ 组明显大于 Ａ 组和 Ｄ 组，６ ｄ 时组间差异不显著，９ ｄ 时 Ｄ 组明显小于 Ａ 组和 Ｂ 组；排便次数，３ ｄ 时和９ ｄ 时组间差异不显著，但６ ｄ 时 Ｄ 组明显大于 Ａ 组和 Ｂ 组。染毒前后自身比较， Ｄ 组错误率染毒后一直升高，９ ｄ 时明显高于染毒前；走格子数， Ａ 组和 Ｂ 组９ ｄ 时明显高于染毒前。Objective To study the effect of low concentration CO on neurobehaviour function and the maximal allowable concentration(MAC) in limited time. Method 40 KM (kunming) male mice were randomly divided into groups A,B,C and D. They were exposed to normal air, 30 mg/m 3 CO, 300 mg/m 3 CO and 600 mg/m 3 CO respectively. The changes in neurobehaviour function of the mice before,during and after 72 h exposure in 0.5 m 3 static chambers were observed. Four indices were measured: response time (RT) and error rate (ER) in the water maze test, enter check frequency (ECF) and urine or excrement frequency (UEF) in open field test. Result The set upright frequency in group D was smaller than those in groups A,B and C during exposure. RT and ER values went in a direction contrary to pre exposure the four groups in the second 3 days (6 d) after or out of exposure, while ER values showed that group D>group C>group A>group B in the third 3 days (9 d).RT value was not different among the 4 groups, but its order of numerical value was similar to the ER order. The order of ECF values was that group C>group A and group D in 3 d after or out of exposure and not different among the 4 groups in 6 d and group D<group A and group B in 9 d. UEF values were not different among the 4 groups in 3 d and 9 d, but group D>group A and group B in 6 d after or out of exposure. Comparison with every group itself, showed statistically significance increase in ER values in 9 d out of exposure than before exposure in group D. ECF values showed the statistics significant increase in 9 d in group A and B. Conclusion Low concentration CO may interfere with the learning and memory of mice in the water maze and impede the development of the motor nervous function and disturb the steadiness of emotion of mice in the open field. In addition, low concentration of CO may be of tardy toxicity.

关 键 词：一氧化碳 神经行为 毒性 小鼠 容许浓度 

分 类 号：R595.1[医药卫生—内科学]