培养胚胎神经干细胞向少突胶质细胞诱导分化的实验研究  

The induction and differentiation of oligodendrocyte from embryonic neural stem cells of rats in vitro

作　　者：王嵩[1] 张丽霞[2] 柴勇[3] 杨成[4] 

机构地区：[1]滨州医学院教务处,烟台市264003 [2]滨州医学院实验医学研究中心,烟台市264003 [3]滨州医学院基础学院 [4]滨州医学院中西医结合学院

出　　处：《滨州医学院学报》2011年第1期26-28,32,共4页Journal of Binzhou Medical University

基　　金：山东省优秀中青年科学家奖励基金(2004BS02017)

摘　　要：目的培养大鼠胚胎神经干细胞,诱导分化获得较高纯度的少突胶质细胞。方法取孕11.5 d的胚胎大鼠神经管尾段制备单细胞悬液,无血清条件培养基培养,获得神经干细胞;加入胎牛血清诱导分化,利用神经元、星形胶质细胞和少突胶质细胞不同的生长特性进行分离,获取较高纯度的少突胶质细胞。结果本实验得到纯度达90%以上的少突胶质细胞。结论利用培养大鼠胚胎神经干细胞诱导分化可以获取较多较高纯度的少突胶质细胞,以进行进一步研究。Objective To culture embryonic neural stem cells of rats and differentiate into oligodendrocytes in vitro,purifing in order to obtain highly purified oligodendrocyte.Methods The cells derived from the posterior segment of neural tube in E11.5d rat were cultured in serum-free conditioned medium and neural stem cells were obtained,which were induced to differentiate into neurons,astrocytes and oligodendrocyte by adding fetal bovine serum.To separate these differentiated cells according to their different physical and chemical characteristics,highly purified oligodendrocytes were gained.Results The purity of oligodendrocytes can be more than 90%.Conclusion Large quantities and high purity of oligodendrocytes were gained by culturing embryonic neural stem cells of rats in vitro,for the purpose of further research and analysis.

关 键 词：神经干细胞 少突胶质细胞 培养 分化 纯化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]