HPLC法测定六味地黄丸熊果酸的含量  

Determination of Ursolic Acid in Liuwei Dihuang Pills by HPLC

作　　者：卢翠文[1] 

机构地区：[1]柳州师范高等专科学校,广西柳州545004

出　　处：《安徽农业科学》2011年第4期2070-2071,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]用高效液相色谱（HPLC）法测定六味地黄丸（浓缩丸）的熊果酸含量,提高熊果酸的质量控制标准。[方法]色谱柱：WelchMaterials AQ-C18柱（5μm,4.6 mm×250.0 mm）;流动相：乙腈-水-磷酸（V∶V∶V=90∶10∶0.1）;柱温：25℃;流速：1.0 ml/min;检测波长为210 nm。[结果]在0.457 6~1.542 3μg/ml的线性范围内,熊果酸与峰面积呈良好的线性关系,平均回收率为99.31%,RSD值为1.09%（n=6）。[结论]高效液相色谱法灵敏、简单、快速、准确,适用于六味地黄丸（浓缩丸）熊果酸含量的测定。[Objective] To determine the content of ursolic acid in liuwei dihuang pills by HPLC,so as to promote the quality control standard of ursoli acid.[Method] HPLC conditions were as follows： column of Welch Materials AQ-C18 column（5 μm,4.6 mm×250.0 mm）;mobile phase of acetonitrile-water-phosphoric acid（V∶ V∶ V=90∶ 10∶ 0.1）;column temperature of 25 ℃;flow rate of 1.0 ml/min and detection wavelength of 210 nm.[Result]The relationship between ursolic acid and peak area was good in the range of 0.457 6~1.542 3 μg/ml,and the mean recovery of ursolic acid was 99.31% with 1.09% in RSD value（n=6）.[Conclusion]Simple in operation,accurate in result,HPLC method is favorable for the determination of ursolic acid in liuwei dihuang pills.

关 键 词：六味地黄丸(浓缩丸) 熊果酸 含量测定 高效液相色谱法 

分 类 号：S567[农业科学—中草药栽培]