稀土Y^(3+)对铜绿微囊藻生长和生理特性的影响  

Effects of Yttrium(Y^(3+))on Physiological and Biochemical Characteristics of Microcystis aeruginosa

作　　者：王应军[1] 陈燕[1] 金航标[1] 高鹏[1] 余游 

机构地区：[1]四川农业大学资源环境学院,四川雅安625014 [2]重庆市巫溪县环保局,重庆405800

出　　处：《安徽农业科学》2011年第4期2248-2251,共4页Journal of Anhui Agricultural Sciences

基　　金：国家级大学生创新计划项目(01409207);四川省教育厅重点资助项目(09ZA064)

摘　　要：[目的]探讨不同浓度钇(Y3+)对铜绿微囊藻(Microcystis aeruginosa)生长特性的影响和藻细胞超微结构的变化。[方法]以铜绿微囊藻FACHB912为试材,采用生理和生化方法研究了不同浓度的外源稀土Y3+(0、0.05、0.10、0.20、0.50、1.00、2.00、5.00和10.00 mg/L)对藻细胞生长的影响,并比较了藻细胞叶绿素a、丙二醛(MDA)的含量及其超微结构随不同浓度Y3+胁迫的变化。[结果]相对低浓度Y3+(0.05~0.20 mg/L)对铜绿微囊藻生长表现出明显促进作用,而高浓度Y3+(0.50~10.00 mg/L)则部分或完全抑制了藻细胞的正常生长;Y3+对铜绿微囊藻的叶绿素a合成、MDA也有影响。叶绿素a随Y3+浓度的提高呈现先上升后下降的变化趋势;低浓度Y3+(0.05~0.20 mg/L)对铜绿微囊藻MDA含量无显著影响,然而随着Y3+浓度的增加和胁迫时间的延长,藻细胞中MDA含量显著上升。[结论]一定低浓度的Y3+可有效促进铜绿微囊藻的生长和藻细胞叶绿素a等生理指标的上升。[Objective]This paper aimed to discuss the influence of different concentrations of initial yttrium（Y3＋） on the physiological and biochemical characteristics of Microcystis aeruginosa.[Method]Taking M.aeruginosa as the test material,some physiological and biochemical methods were used to study the impact of Y3＋ with various concentration（0,0.05,0.10,0.20,0.50,1.00,2.00,5.00 and 10.00 mg/L,respectively） on the growth of M.aeruginosa.Also,the Chl-a,MDA content and ultrastructure changes in Y3＋ treated algaes were compared.[Result]Relatively low concentrations of Y3＋（0.05-0.20 mg/L） had auxo-actions to the growth of M.aeruginosa,while a higher concentrations of Y3＋（0.50-10.00 mg/L） could partly,even completely,inhibit the growth of M.aeruginosa.Comparing with the control,the Chl-a content was in the trendency of increasing firstly,and then it decreased when the initial Y3＋ concentrations increased from 0.05 to 5.00 mg/L.When the M.aeruginosa was exposed to Y3＋ concentration of 0.05-0.20 mg/L,the significant variation in the MDA content was not observed.However,it enhanced sharply when the Y3＋concentration increased from 1.00 to 5.00 mg/L.[Conclusion] The rare earth Y3＋ at some content could effectively stimulate the growth of M.aeruginos and promote the rising of the physiological indexes such as Chl-a content in alga cells.

关 键 词：钇 铜绿微囊藻 叶绿素A 丙二醛 超微结构 

分 类 号：X172[环境科学与工程—环境科学]