放疗联合热疗诱导肝癌HepG2细胞凋亡及其与Bcl-2和Bax蛋白表达关系的研究  

Apoptosis of Hepatoma Cell Line HepG2 Induced by the Combination of Radiotherapy and Thermotherapy and Its Relationship with Bcl-2/Bax Protein Expressions

作　　者：张力[1] 龚明玉[2] 李毅学[1] 张立广[1] 王兴艳[1] 

机构地区：[1]承德医学院附属医院肿瘤放化疗科,河北省承德市067000 [2]承德医学院生物化学教研室

出　　处：《中国全科医学》2011年第6期627-630,共4页Chinese General Practice

摘　　要：目的探讨放疗联合热疗诱导人肝癌HepG2细胞凋亡及其与Bcl-2和Bax蛋白表达的关系。方法将体外培养的肝癌HepG2细胞随机分为未治疗对照组、单纯放疗组、单纯热疗组、放疗联合热疗组,采用流式细胞术检测细胞的凋亡情况,免疫组化法检测细胞凋亡相关基因Bcl-2和Bax蛋白表达水平。结果 4组肝癌HepG2细胞凋亡率比较,差异有统计学意义(P<0.05);其中单纯热疗组、单纯放疗组及放疗联合热疗组较未治疗对照组明显升高,差异均有统计学意义(P<0.05);放疗联合热疗组较单纯热疗组和单纯放疗组明显升高,差异均有统计学意义(P<0.05)。4组肝癌HepG2细胞凋亡相关基因Bcl-2、Bax蛋白表达和Bax/Bcl-2比较,差异均有统计学意义(P<0.05);其中单纯热疗组、单纯放疗组及放疗联合热疗组较未治疗对照组Bcl-2蛋白阳性表达率明显降低,Bax蛋白阳性表达率明显升高,Bax/Bcl-2明显升高,差异均有统计学意义(P<0.05);放疗联合热疗组较单纯热疗组和单纯放疗组Bcl-2蛋白阳性表达率明显降低,Bax蛋白阳性表达率明显升高,Bax/Bcl-2明显升高,差异均有统计学意义(P<0.05)。结论放疗联合热疗能更有效地诱导肿瘤细胞凋亡,其机制可能是通过抑制Bcl-2蛋白的表达、增加Bax蛋白的表达来实现。Objective To explore the apoptosis of hepatoma cell line HepG2 induced by the combination of radiotherapy and thermotherapy and its relationship with Bcl-2/Bax protein expressions.Methods In vitro cultured HepG2 cells were randomly divided into four groups：control group（not treated）,radiotherapy group,thermotherapy group,and combination group.The apoptosis of HepG2 cells were detected by flow cytometry.The expressions of the apoptosis-related proteins of Bcl-2 and Bax were detected by immunohistochemical methods.Results The apoptosis rates of HepG2 cells were significantly different among these four groups（P0.05）.The apoptosis rates were significantly higher in radiotherapy group,thermotherapy group,and combination group than in control group（P0.05）.It was also significantly higher in combination group than in radiotherapy group and thermotherapy group（P0.05）.The expressions of Bcl-2 and Bax and the Bax/Bcl-2 ratio were also significantly different among these four groups（P0.05）.The expression of Bcl-2 protein were significantly decreased and the expression of Bax protein significantly increased in radiotherapy group,thermotherapy group,and combination group than in control group（both P0.05）,and the Bax/Bcl-2 ratio was also significantly increased（P0.05）.The expression of Bcl-2 protein were significantly decreased and the expression of Bax protein significantly increased in combination group than in radiotherapy group and thermotherapy group（both P0.05）,and the Bax/Bcl-2 ratio was also significantly increased（P0.05）.Conclusion The combination of radiotherapy and thermotherapy can more effectively induce the apoptosis of HepG2,and it may be achieved by inhibiting the expression of Bcl-2 protein and promoting the expression of Bax protein.

关 键 词：肝肿瘤 细胞凋亡 放射疗法 

分 类 号：R735.7[医药卫生—肿瘤]