辛伐他汀对变态反应性脑脊髓炎小鼠INF-γ和IL-10表达的影响  

Effect of simvastatin on expression of INF-γ and IL-10 in mice with allergic encephalomyelitis

作　　者：曹江晨 雷军平[2] 李路军[3] 陈光勇[4] 

机构地区：[1]解放军73801部队门诊部 [2]第二军医大学附属长海医院心内科 [3]南京军区联勤部无锡干休所卫生所 [4]南京军区福州总医院第一附属医院

出　　处：《东南国防医药》2011年第1期24-27,共4页Military Medical Journal of Southeast China

摘　　要：目的研究辛伐他汀(Simvastatin)对实验性变态反应性脑脊髓炎(experiment allergic en-cephalomyelitis,EAE)小鼠肿瘤坏死因子-γ(INF-γ)和白细胞介素-10(IL-10)表达的影响,探讨该药物对EAE保护作用的免疫调节机制。方法通过皮下注射MOG33-55肽段诱导小鼠急性EAE模型,实验分为EAE给药组、EAE对照组和正常对照组。观察各组小鼠体重等变化,测定EAE发病高峰期血清、培养的单核细胞及脾细胞培养上清液的INF-γ和IL-10水平。结果辛伐他汀能降低EAE小鼠的发病率,减轻了症状的严重程度,并能显著降低EAE小鼠血清、单个核细胞及脾细胞培养上清中INF-γ水平,升高IL-10水平。结论辛伐他汀可能通过其抑制INF-γ和上调IL-10的表达,调节免疫状态,从而对小鼠EAE起到保护作用。Objective To investigate the effect of simvastatin on expression of INF-and IL-10 in mice with experiment allergic encephalomyelitis（EAE）,and the immunomodulatory mechanism for protection of allergic encephalomyelitis.Methods EAE was induced in C57BL/6J mice by immunization with MOG33-55.Mice were randomly divided into three groups： EAE simvastatin treatment group,EAE sham treatment group,and normal control group.The clinical assessment of EAE was performed daily.The levels of INF-γ and IL-10 in the serum,and the cell suspension of cultured mononuclear cell（MNC） or spleen cell were analyzed by ELISA.Results Simvastatin reduced the incidence and severity of EAE,which was accomplished by the inhibited expression of INF-γ and enhanced expression of IL-10.Conclusions Simvastatin might ameliorated EAE through an immunomodulatory effect that inhibits the expression of INF-γ and enhances the expression of IL-10.

关 键 词：多发性硬化 实验性变态反应性脑脊髓炎(EAE) 辛伐他汀 INF-Γ IL-10 

分 类 号：R744.5[医药卫生—神经病学与精神病学]